Sox2和Oct4在人脑胶质瘤组织中的表达及意义  

The expression and significance of Sox2 and Oct4 in human glioma tissue

作　　者：王博[1] 唐景峰[2] 黄永旺[2] 杨保华[2] 郑华平[2] 毕洪伟[2] 

机构地区：[1]桂林医学院研究生院,桂林541004 [2]桂林医学院附属医院神经外科,桂林541004

出　　处：《中国当代医药》2015年第9期18-21,共4页China Modern Medicine

摘　　要：目的探讨Sox2和Oct4在不同病理级人脑胶质瘤组织中的表达及意义。方法采用免疫组织化学技术及组织芯片检测Sox2和Oct4在45例人脑胶质瘤组织和5例正常人脑组织中的表达。结果 Sox2在正常人脑组织中的表达率为(2.61±1.33)%,结果为阴性,在人脑胶质瘤组织中WHOⅠ、Ⅱ、Ⅲ、Ⅳ级的阳性表达率分别为(3.95±3.40)%、(18.22±5.16)%、(20.74±5.14)%、(60.15±7.82)%,Sox2在正常人脑组织中和人脑胶质瘤组织中的表达比较,差异有统计学意义(F=87.9,P<0.01),Ⅰ、Ⅱ级与Ⅲ、Ⅳ级比较,差异有统计学意义(P<0.01);Oct4在正常人脑组织中完全不表达,在人脑胶质瘤组织中WHOⅠ、Ⅱ、Ⅲ、Ⅳ级的阳性表达率分别为(1.25±3.82)%、(37.20±9.12)%、(51.39±9.72)%、(63.15±9.05)%,Oct4在正常人脑组织中和人脑胶质瘤组织中的表达比较,差异有统计学意义(F=67.7,P<0.01),Ⅰ、Ⅱ级与Ⅲ、Ⅳ级比较,差异有统计学意义(P<0.01);Sox2和Oct4的表达强度均随病理级别增高而升高,而在正常人脑组织中均未见表达;在人脑胶质瘤组织中Sox2和Oct4的表达呈正相关(r=0.619,P<0.01);不同性别、年龄的人脑胶质瘤组织中Sox2、Oct4的阳性表达率差异均无统计学意义(P>0.05)。结论 Sox2和Oct4在人脑胶质瘤组织中的表达与肿瘤的恶性程度呈正相关,提示Sox2和Oct4可能在人脑胶质瘤的发生、发展中起重要作用,其在人脑胶质瘤组织中的高表达可能是人脑胶质瘤恶性程度高、呈侵袭性生长的原因之一。Objective To explone the expression and significance of Sox2 and Oct4 in human glioma tissue of different pathological grade. Methods Immunohistochemical technique and tissue microarray were used to detect the expression of Sox2 and Oct4 in 45 cases of human glioma tissue and 5 cases of normal brain tissue. Results The expression rate of Sox2 was（2.61±1.33）% in normal human brain tissue,the results were negative,and the positive expression rate of Sox2 in human glioma tissue of WHO Grade Ⅰ,Ⅱ,Ⅲ,Ⅳ was（3.95%±3.40）%,（18.22%±5.16）%,（20.74%±5.14）%,（60.15%±7.82）% respectively,and there was a statistical difference of the expression of Sox2 in normal human brain tissue and human glioma tissue（F=87.9,P〈0.01）,there was a statistical difference of betweent the grade Ⅰ,Ⅱ and grade Ⅲ,Ⅳ（P〈0.01）.Oct4 in normal human brain tissue had no expression,the positive expression rate of Oct4 in human glioma tissue of WHO Grade Ⅰ,Ⅱ,Ⅲ,Ⅳ was（1.25±3.82）%,（37.20±9.12）%,（51.39±9.72）%,（63.15±9.05）% respectively,and there was a statistical difference of the expression of Oct4 in normal human brain tissue and human glioma tissue（F=67.7,P〈0.01）,there was a statistical difference of betweent the grade Ⅰ, Ⅱ and grade Ⅲ, Ⅳ（P〈0.01）.The expression intensity of Sox2 and Oct4 were increased with the pathological grade elevating,while in normal human brain tissue showed no expression;Sox2 expression in human glioma tissue was positively correlated with that of Oct4（r=0.619,P〈0.01）.There was no statistical difference of the positive expression rate of different sex and age in human glioma tissue（P〉0.05）. Conclusion The expression of Sox2 and Oct4 in human glioma tissue is positively related with the malignant degree of the tumor and it is suggested that Sox2 and Oct4 may play an important role in the occurence and development of the human glioma tis-sues and its high expressions in human glioma tissue may be one of the reasons of higher degree

关 键 词：胶质瘤 SOX2 OCT4 组织芯片 免疫组织化学 

分 类 号：R739.41[医药卫生—肿瘤]