咪唑离子液体对四尾栅藻的毒性  

Toxicity of imidazolium- based ionic liquids to Scenedesmus quadricauda

作　　者：吴洁[1,2] 陈晓娣[1,2] 邓芸[2,3] 朱凯[2,3] 韩萍芳[1] 顾军[2,3] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京大学昆山创新研究院,江苏昆山215347 [3]南京大学物理学院,江苏南京210093

出　　处：《生物加工过程》2015年第2期76-80,共5页Chinese Journal of Bioprocess Engineering

基　　金：江苏省自然科学基金(BK2012218)

摘　　要：按照藻类生长抑制试验标准方法,以四尾栅藻为受试生物,研究了离子液体1-丁基-3-甲基咪唑氯盐([C4mim][Cl])和1-丁基-3-甲基咪唑四氟硼酸盐([C4mim][BF4])对四尾栅藻的影响,测定了半数有效浓度(EC50)和叶绿素含量。结果表明:[C4mim][Cl]和[C4mim][BF4]对四尾栅藻的生长和叶绿素的产生均具有抑制作用,毒性效应随着浓度的增大和培养时间的增长而增加,96 h的EC50分别为57.8和38.8 mg/L。离子液体质量浓度为200mg/L时,四尾栅藻的叶绿素总量仅为对照组的32%和26%。此结果可以说明离子液体对生态系统存在潜在的负面效应。Toxic effects of ionic liquids 1-butyl-3-methylimidazolium chloride（[C4mim][Cl]） and 1-butyl-3-methylimidazolium tetrafluoroborate（[C4mim][BF4]） on Scenedesmus quadricauda were studied by using alga growth inhibition test.Two endpoints EC50 values and chlorophyll contents were determined.The results indicate that [C4mim][Cl] and [C4mim][BF4]limited the growth and chlorophyll content of S.quadricauda,the toxic effects increased with the concentration and incubation time.The 96 h EC50 was 57.8 and 38.8 mg/L,respectively.When adding 200mg/L ionic liquid,the total chlorophyll content of S.quadricauda were only 32% and 26% respectively,compared with the control group.Our findings imply the potential adverse effect of ionic liquids on the ecological system.

关 键 词：四尾栅藻 离子液体 毒性 半数有效浓度EC50 叶绿素含量 

分 类 号：Q93[生物学—微生物学]