消栓通络有效成分组对大鼠脑缺血损伤的保护作用及其机制  

Protective effects and mechanism of effective components group of Xiaoshuantongluo on cerebral ischemia injury in rats

作　　者：谢欣梅[1] 王琳[1] 赵艳[2] 陈若芸[3] 庞晓斌[1] 杜冠华[3] 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]青岛市市立医院,山东青岛266011 [3]中国医学科学院&北京协和医学院药物研究所国家药物筛选中心,北京100050

出　　处：《河南大学学报（医学版）》2015年第1期6-9,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金资助项目(81273652)

摘　　要：目的研究消栓通络有效成分组(XECG)对大鼠脑缺血再灌注(I/R)损伤的保护作用及其机制。方法SD大鼠随机分为6组:假手术组,模型组,尼莫地平对照组(4 mg·kg-1),XECG高、中、低剂量组(200、100、50mg·kg-1)。采用线栓法阻断大脑中动脉(MCAO)建立大鼠脑缺血/再灌注(I/R)模型。缺血2h,再灌注24h后,HE染色观察大鼠缺血侧脑组织的病理变化;Western blot法检测缺血侧脑组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(Interleukin 1β,IL-1β)的表达。结果 XECG能明显改善缺血再灌注大鼠脑组织的病理变化,降低缺血侧脑组织TNF-α、IL-1β的表达。结论 XECG对脑缺血再灌注大鼠具有明显的保护作用,其机制可能与降低TNF-α、IL-1β的表达,抑制炎症级联反应有关。Objective To observe the protective effect and mechanism of effective components group of Xiaoshuantongluo（XECG）on ischemia/reperfusion（I/R）injury in rats.Methods Healthy male adult Sprague Dawley rats were randomly divided into the sham operation group,the model group,the nimodipine（4mg·kg-1）group,and high,middle and low dose XECG groups（200,100,50mg·kg-1）.Middle cerebral artery occlusion was adopted to establish the rat ischemia/reperfusion model.After ischemia for 120 min and reperfusion for 24 h,the pathological injury of the ischemia side was observed by HE staining.Expressions of tumor necrosis factorα（TNF-α）,Interleukin 1β（IL-1β）were detected by western blotting technique.Results XECG could significantly improve the pathological changes of cortical penumbra brain tissues,Compared with the I/R model group,XECG could significantly inhibit expressions of TNF-α、IL-1β.Conclusion XECG has obviously protective effect on Sprague Dawley rats after cerebral ischemia and reperfusion,its mechanisms may be associated with inhibiting inflammatory,expression of TNF-αand IL-1βproteins.

关 键 词：XECG 脑缺血/再灌注 炎症反应 

分 类 号：R743.3[医药卫生—神经病学与精神病学]