中药黄芩苷对牙髓干细胞增殖及成牙成骨分化能力的影响  

Effect of baicalin on the proliferation and odento/osteogenic differentiation of human dental pulp stem cells

作　　者：薛晶[1] 俞艳[1,2] 王利娟[3] 于金华[1,2] 张光东[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院牙体牙髓病科,江苏南京210029 [3]常州市口腔医院牙体牙髓病科,江苏常州213003

出　　处：《口腔生物医学》2015年第1期11-14,共4页Oral Biomedicine

基　　金：江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的：探讨中药黄芩苷对牙髓干细胞的增殖、成牙/成骨分化能力的影响。方法：酶消化法原代培养人牙髓干细胞,采用甲基噻唑基四唑（MTT）比色法检测黄芩苷对人牙髓干细胞增殖的影响,碱性磷酸酶活性检测及Western blot等方法检测黄芩苷作用于人牙髓干细胞后,其成牙/成骨分化指标,包括核心结合因子（runt-related transcription factor 2,RUNX2）、牙本质涎蛋白（dentin sialoprotein,DSP）、Osterix（OSX）、骨桥蛋白（osteopontin,OPN）、骨钙素（osteocalcin,OCN）的变化。结果：MTT检测结果显示,0~20μmol/L黄芩苷作用于牙髓干细胞后其增殖能力与对照组相比无明显差异（P〉0.05）;而碱性磷酸酶活性检测显示：0~20μmol/L黄芩苷各浓度组细胞ALP活性比对照组明显增加（P〈0.01）;Western blot结果表明：与对照组相比,20μmol/L黄芩苷可增强牙髓干细胞RUNX2、DSP、OSX、OPN、OCN等成牙/成骨相关蛋白的表达。结论：黄芩苷对牙髓干细胞的增殖无明显影响,但可促进其成牙/成骨分化能力。Objective： To investigate the effect of baicalin on the proliferation and odento/osteogenic differentiation of huaman dental pulp stem cells（ h DPSCs） in vitro. Methods： h DPSCs were isolated from dental pulp and then were cultured in alpha minimun essential medium（ α-MEM） supplimented with or without baicalin. MTT colorimetric method was used to examine the proliferation ability of h DPSCs. The effect of baicalin on the odento / osteogenic differentiation was observed by alkaline phosphotase（ ALP） activity and Western blot. Results： MTT assay showed that there was no significant difference between baicalin-treated and untreated control cells（ P 〉0. 05）,and ALP activity assay demonstrated that 0 ~ 20 μmol / L baicalin enhanced the ALP activity of DPSCs（ P 〈0. 01）,and Western blot revealed that the odento / osteogenic marker（ RUNX2,DSP,OSX,OPN,OCN） was significantly upregulated in baicalin-treated cells compared with the untreated cells. Conclusions： Baicalin has no effect on the proliferation of DPSCs,but enhances the capacity of odento / osteogenic differentiation.

关 键 词：牙髓干细胞 黄芩苷 增殖 分化 

分 类 号：R780.2[医药卫生—口腔医学]