Rictor在贲门癌组织中的表达及临床意义  

Expressions and Clinical Significances of Rictor in Gastric Cardiac Cancer

作　　者：张鹏飞[1] 刘永萱 张亚利[1] 马志坤[1] 梁硕[1] 冯笑山[1] 

机构地区：[1]河南科技大学第一附属医院,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2015年第1期9-13,共5页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的检测Rictor在贲门癌及相应癌旁正常组织中的表达差异,分析其与贲门癌临床病理之间及预后的关系,判断其作为贲门癌早期诊断及预后指标的可能性。方法采用荧光定量PCR方法、免疫组织化学S-P法检测Rictor在30例贲门癌组织及相应癌旁正常组织中的表达情况。结果贲门癌癌旁组织中Rictor基因拷贝数为9.10×10^8-11.12×10^9,贲门癌组织中为7.78×10^5-8.58×10^6;Rictor在贲门癌组织及相应癌旁正常组织中的阳性表达率分别为56.7%（17/30）、76.7%（23/30）,统计学处理有显著性差异（P〈0.05）。Rictor在贲门癌组织中表达降低与患者的年龄、性别、TNM分期及淋巴结转移均无关（P〉0.05）,与肿瘤分化程度相关（P〈0.05）。结论 Rictor在贲门癌旁组织中的表达明显高于贲门癌组织中的表达,初步推断该基因的表达与癌症进程相关,有可能成为贲门癌早期诊断、治疗以及预测贲门癌患者预后的具有重要应用价值的指标。Objective To detect the expressions of Rictor in gastric cardiac adenocarcinoma（ GCA） and corresponding adjacent normal tissues,analyze the relationship between it and clinical pathological features and prognosis of gastric cardia cancer,judge its possibility as early diagnosis and prognostic index of gastric cardia cancer. Methods The expressions of Rictor in 30 cases of cardiac carcinoma tissues and 30 cases of the corresponding adjacent normal tissues were measured by the fluorescent quantitative PCR method and immunohistochemistry S-P method. Results Rictor gene copy number of adjacent tissues was 9. 10 × 10^8- 11. 12 × 10^9. GCA was 7. 78 × 10^5- 8. 58 × 10^6. The expression of Rictor protein in the corresponding adjacent normal tissues was 76. 7%（ 23 /30）,and the GCA tissue was 56. 7%（ 17 /30）. There existed significant difference between them（ P〈0. 05）. There were no significant correlations of the decreased expression of Rictor with the age,sex,TNM and lymph node metastasis（ P〉0. 05）,but tumor differentiation（ P〈0. 05）. Conclusion The expression levels of Rictor in adjacent tissues is much higher than those in the GCA tissues（ P〈 0. 01）. Preliminary inference of the expression of Rictor is related with the progression of cancer,therefore,it may be used in the early diagnosis,therapy and prognosis in GCA.

关 键 词：贲门癌 RICTOR 荧光定量PCR 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]