全反式维甲酸联合索拉菲尼对人SMMC-7721肝癌细胞株的作用  

Combination effect of SMMC-7721 hepatocarcinoma cells in vitro treated with all-trans retinoic acid and sorafenib

作　　者：陈彦明[1] 张炳勇[1] 张继承[2] 王晶晶[2] 李子浩[2] 李永真[2] 

机构地区：[1]郑州大学人民医院消化内科,郑州450003 [2]郑州大学第二附属医院肿瘤科,郑州450014

出　　处：《医药论坛杂志》2015年第2期44-46,49,共4页Journal of Medical Forum

摘　　要：目的观察全反式维甲酸联合索拉菲尼对SMMC-7721细胞增殖及凋亡的影响。方法选用ATRA(10-5mol/L)及不同浓度的索拉菲尼(6μmol/L、9μmol/L、12μmol/L),并选用10-5mol/L的ATRA分别联合索拉菲尼(6μmol/L、9μmol/L、12μmol/L)作用于SMMC-7721肝癌细胞24h、48h、72h;期间药物对SMMC-7721肝癌细胞生长的抑制阻滞作用通过MTT比色法进行观测,同时细胞生长过程中利用倒置显微镜观察形态变化,其后药物作用48h时SMMC-7721细胞的周期分布和凋亡的情况利用流式细胞术分析。结果不同浓度的索拉菲尼单药、全反式维甲酸单药及联合用药均对SMMC-7721肝癌细胞的生长明显抑制并改变细胞的形态,促进肝癌细胞的凋亡比例增加。其凋亡作用有随剂量-时间改变的依赖性。两药联合应用相比单用全反式维甲酸或索拉菲尼其细胞凋亡作用更为显著。12μmol/L索拉菲尼、9μmol/L索拉菲尼分别与ATRA联合作用于细胞48h及72h相比较,其抑制作用无明显差异。两药联合作用时可见细胞周期阻滞在S期的肝癌细胞较单药应用时显著增多(P<0.01),凋亡率增加。结论 ATRA、索拉菲尼均有阻滞肝癌SMMC-7721细胞增殖及促凋亡的作用,联合索拉菲尼作用增强并具协同作用。Objective To observe the proliferation of hepatocarcinoma SMMC- 7721 cells be treated with All- trans retinoic acid（ ATRA） and various concentration of sorafenib. Methods Hepatocarcinoma cells was treated by ATRA（ 10-5mol / L） and / or sorafenib（ 6μmol / L、9μmol / L、12μmol / L） at 24 h,48h and 72 h. Get the assessment on cellular proliferation be inhibited by ATRA and sorafenib in MTT assay. And utilize the inverted microscope to observe the morphological changes of these cells. After that,the progression of cells and the induction of apoptosis could be detected in flow cytometry when cells were treated for 48 h. Results Sorafenib of different preparated concentration and ATRA in monotherapy group or combination treatment group both can inhibit hepatocarcinoma SMMC- 7721 cells＇ growth,and cells＇ morphology shifted,induction of apoptosis increased,follow the dose and time- dependent way. The combination treatment on hepatocarcinoma cells get significantly effect compared to used ATRA or sorafenib separately,and in the combination group more cells blocked at S phase（ P〈0. 01）. No statistical difference showed with 12μmol / L sorafenib compared to 9μmol / L sorafenib combined with ATRA in 48 h and 72 h. Conclusion Combined utilization of the ATRA and sorafenib showed synergistic effect on the proliferation of hepatocarcinoma SMMC- 7721 cells be inhibited,and the apoptosis increased.

关 键 词：全反式维甲酸 索拉菲尼 肝细胞癌 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]