Hes1在大鼠脊髓发育不同阶段的表达  

Expression of the Hes1 in the development process of rat spinal cord

作　　者：马杰[1] 江金群[2] 胡建国[3] 张玉心[4] 石莹[4] 

机构地区：[1]蚌埠医学院临床检验诊断学实验中心,安徽蚌埠233030 [2]蚌埠医学院粤北人民医院检验科,广东韶关512026 [3]蚌埠医学院组织移植省重点实验室,安徽蚌埠233030 [4]蚌埠医学院医学检验系,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2015年第3期314-317,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(81071268);蚌埠医学院科研课题(BYKY1204)

摘　　要：目的:检测Hes1在大鼠脊髓发育过程中不同阶段的表达。方法:选取胚胎15.5 d、18.5 d和出生当天,出生后3 d、7 d、2周、4周及成年的SD大鼠脊髓,采用Western blot和荧光定量PCR检测Hes1在大鼠脊髓发育过程中的表达。结果:从胚胎15.5 d到成年期,大鼠脊髓中都有Hes1的表达,具有一定的变化规律,总体上来看,胚胎期表达量比较高,出生后的表达降低。结论:Hes1在大鼠脊髓发育过程中的不同阶段表达量有所不同,胚胎期表达量高,出生后表达量明显下降。Objective： To detect the expression of Hes1 in the development process of rat spinal cord. Methods： The rat spinal cord at embryonic day 15. 5 and 18. 5,the day of birth,3 days after birth,7 days,2 weeks and 4 weeks,and adult rat spinal cord were harvested,the Hes1 expressions of which were detected by the fluorescence quantitative PCR and Western blot. Results： The expressions of Hes1 in the rat spinal cord of 15. 5 d to adulthood were found,which showed certain regular changes. The expressions of Hes1 in embryonic stages of rat was high,which was decreased after birth. Conclusions： The expression of Hes1 at different stages of the rat spinal cord development is different,which was high in the embryonic period,and was depressed after birth.

关 键 词：hes1基因 脊髓 大鼠 

分 类 号：R737.33[医药卫生—肿瘤]