直链烷基苯磺酸钠对小鼠皮肤组织的氧化应激损伤及大豆异黄酮的拮抗作用研究  

Effect of linear alkylbenzenesulfonate on the oxidative stress damage of mouse skin and antagonism of isoflavone

作　　者：赵文红[1] 张建国[2] 王开磊[1] 张雯[1] 王亚军[1] 王金花[1] 

机构地区：[1]蚌埠医学院预防医学系,安徽蚌埠233030 [2]蚌埠医学院第一附属医院麻醉科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2015年第3期322-325,共4页Journal of Bengbu Medical College

基　　金：安徽省高等学校省级自然科学研究重点资助项目(KJ2014A159);安徽省高校预防医学特色专业资助项目(皖教高[2011]5号);蚌埠医学院教学研究资助项目(jyxm1358)

摘　　要：目的：探讨不同剂量直链烷基苯磺酸钠（LAS）对小鼠皮肤组织氧化应激的影响及大豆异黄酮的拮抗作用。方法：将40只昆明种雄性小鼠随机分成4组,每组10只,分别为正常对照组、LAS低剂量组（LAS 150 mg/L）、中剂量组（LAS 300 mg/L）和高剂量组（LAS 600 mg/L）。用蒸馏水及不同剂量的LAS涂抹小鼠的背部剃毛处,连续涂抹60 d。将50只小鼠随机分为5组,分别为正常对照组、LAS氧化应激损伤组（LAS 300 mg/L）及3个拮抗组（分别用50、100及150 mg/L大豆异黄酮涂抹,再用300 mg/L LAS涂抹）,每天1次,连续涂抹60 d。取涂抹部位皮肤,剪碎制匀浆,检测其中丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性。结果：小鼠皮肤经不同剂量LAS处理后,MDA的含量均明显高于对照组（P〈0.01）,LAS高剂量组的MDA含量均明显高于低剂量组和中剂量组（P〈0.01）;LAS中、高剂量组SOD和GSH-Px的活性均明显低于对照组（P〈0.01）,且随LAS剂量增加,酶的活性逐渐降低（P〈0.01）。大豆异黄酮干预后,各拮抗组MDA含量均低于LAS氧化应激损伤组（P〈0.05~P〈0.01）,但均高于对照组（P〈0.01）;除拮抗组1与LAS氧化应激损伤组GSH-Px活性差异无统计学意义（P〉0.05）外,各拮抗组SOD和GSH-Px活性均高于LAS氧化应激损伤组（P〈0.01）,但均低于对照组（P〈0.05~0.01）;随异大豆黄酮浓度的增加,拮抗效果增加。结论：LAS可导致小鼠皮肤组织氧化应激损伤,大豆异黄酮在一定剂量范围内可以抑制这种应激损伤。Objective： To investigate the effects of linear alkylbenzenesulfonate（ LAS） om the oxidative stress damage of mouse skin and antagonism of isoflavone. Methods： Forty healthy male Kunming mice were randomly divided into the control group,low-dosage LAS group（ 150 mg / L）,middle-dosage LAS group（ 300 mg / L） and high-dosage LAS group（ 600 mg / L）（ 10 mice each group）. The distilled water and different dosage LAS were smeared on the back skin of mice once a day for 60 days. Fifty mice were randomly divided into the control group,LAS oxidative stress damage model group（ skin smeared with 300 mg / L of LAS） and three antagonistic groups（ skin smeared with 300 mg / L LAS after smearing 50,100 and 150 mg / L of isoflavone,once a day for 60 days）. The content of malondialdehyde（ MDA） and activities of super oxidase dimutase（ SOD） and glutathione peroxidase（ GSH-Px） in all mice skin were detected. Results： The content of MDA in mice skin smeared with LAS was remarkably higher than that in control group（ P〈0. 01）,and the contents of MDA in high-dosage LAS group were significantly higher than that in low- and middle-dosage LAS groups（ P〈0. 01）. The activities of SOD and GSH-Px in middle-and high-dosage LAS group were obviously lower than those in control group（ P〈0. 01）,which decreased with the dosage increasing of LAS（ P〈0. 01）. After isoflavone intervention,the contents of MDA in all antagonistic groups were lower than that in LAS damage group,and higher than that in control group（ P〈0. 05 to P〈0. 01）. Except the antagonistic group 1,the differences of the GSH-Px activity between other antagonistic groups and LAS damage groups were not statistically significant（ P〉0. 05）,the activities of SOD and GSH-Px in antagonistic groups were higher than those in LAS damage groups,and lower than those in control group（ P〈0. 05 to P〈0. 01）. The antagonistic effects increased with the isoflavone concentration increasing. Conclusions： LAS can l

关 键 词：氧化应激 直链烷基苯磺酸钠 皮肤组织 大豆异黄酮 拮抗作用 

分 类 号：R363[医药卫生—病理学]