动脉灌注化疗对大鼠脑胶质瘤的治疗作用及其机制  

Therapeutic effect of arterial infusion chemotherapy on glioma of rats and its mechanism

作　　者：王昆鹏[1,2] 赵文清[2,3] 王维兴[1] 呼铁民[1] 康进生[3] 孙鑫 鞠智卿[5] 

机构地区：[1]承德医学院附属医院神经外科,河北承德067000 [2]河北医科大学研究生院,河北石家庄050017 [3]河北省人民医院神经外科,河北石家庄050051 [4]围场满族蒙古族自治县县医院神经外科,河北承德068450 [5]承德医学院附属医院康复科,河北承德067000

出　　处：《吉林大学学报（医学版）》2015年第2期327-332,I0005,共7页Journal of Jilin University：Medicine Edition

基　　金：河北省承德市科技局科技支撑计划项目资助课题(201422029)

摘　　要：目的:探讨动脉灌注化疗对大鼠脑胶质瘤的治疗作用及其机制,为临床应用提供依据。方法:24只脑胶质瘤模型大鼠随机分为动脉组、静脉组和对照组,每组8只,分别通过大鼠尾静脉(静脉组)及颈动脉(动脉组)给予化疗药物VM-26,对照组大鼠给予生理盐水。观察各组大鼠肿瘤体积和生存期;给予不同途径治疗1周后处死大鼠,采用PCR法观察大鼠肿瘤细胞中增殖细胞核抗原(PCNA)和拓扑异构酶Ⅱ(TOPOⅡ)mRNA的表达水平;免疫组织化学法检测大鼠肿瘤细胞中PCNA的阳性细胞数;流式细胞术检测大鼠肿瘤细胞凋亡率。结果:动脉组大鼠经治疗后肿瘤生长速度最慢,静脉组其次,对照组最快,动脉组大鼠肿瘤体积小于静脉和对照组(P<0.05),静脉组大鼠肿瘤体积小于对照组(P<0.05)。与对照组比较,动脉组和静脉组大鼠生存期延长(P<0.05);动脉组大鼠生存期长于静脉组,但组间比较差异无统计学意义(P>0.05)。动脉组大鼠肿瘤组织中PCNA及TOPOⅡmRNA表达水平低于对照组和静脉组(P<0.05),静脉组大鼠肿瘤组织中PCNA及TOPOⅡmRNA表达水平低于对照组(P<0.05)。动脉组大鼠肿瘤组织中PCNA阳性细胞数低于静脉组和对照组(P<0.05)。动脉组大鼠肿瘤细胞凋亡率高于静脉组和对照组(P<0.05)。结论:动脉灌注途径化疗使肿瘤细胞增殖速度减慢,肿瘤细胞凋亡明显增多,能更有效控制肿瘤生长。Objective To study the therapeutic effect and mechanism of arterial infusion chemotherapy on glioma of the rats,and to provide the basis for clinical application.Methods 24 rats were randomly divided into artery group,vein group,and control group;there were 8rats in each group.The chemotherapy medicine VM-26 were injected through tail intravenous（vein group）and carotid arteries（artery group）into the rats,respectively,and the rats in control group were given normal saline.The tumor size and survival period of the rats were observed;all the rats were killed after giving different treatment for one week,and the expression levels of proliferating cell nuclear antigen（PCNA）and topoisomeraseⅡ（TOPO Ⅱ）in the tumor cells of the rats were detected by PCR method,meanwhile the number of PCNA positive cells in the tumor cells were detected by immunohistochemical method;the apoptotic rates of the tumor cells of the rats were detected by flow cytometry method.Results After treatment,the tumor growth speed of the rats in artery group was the slowest,which was followed by vein group,and the speed of the rats in control group was the fastest.The tumor size of the rats in artery group was smaller than those in vein and control groups,and the tumor size of the rats in vein group was smaller than that in control group（P〈0.05）.Compared with control group,the survival period of the rats in artery and vein groups were extended（P〈0.05）;the survival period of the rats in artery group was longer than that in vein group,but there was no statistically significant difference between two groups（P〈0.05）.The expression levels of PCNA and TOPO Ⅱ mRNA in the tumor tissue of the rats in artery group were lower than those in vein and control groups（P〈0.05）,and the expression levels of PCNA and TOPO Ⅱ in the tumor tissue of the rats in vein group were lower than those in control group（P〉0.05）.The number of PCNA positive cells in the tumor tissue of the the rats in artery group was lower than those

关 键 词：脑胶质瘤 动脉灌注 细胞核增殖抗原 拓扑异构酶-Ⅱ 细胞凋亡 化学疗法 

分 类 号：R361.3[医药卫生—病理学]