舌鳞状细胞癌组织中Cks1、Skp2和p27^(kip1)蛋白的表达及其临床意义  

Expressions of Cks1,Skp2,and p27^(kip1) proteins in tongue squamous cell carcinoma tissue and their clinical significances

作　　者：段秀梅[1] 李烁烯 明智慧[2] 张丛笑[2] 刘文书[2] 

机构地区：[1]吉林大学第一医院临床病理诊断中心,吉林长春130021 [2]吉林大学第一医院口腔科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第2期347-351,I0006,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省发改委科研计划项目资助课题(JF2012C006-8)

摘　　要：目的:检测舌鳞状细胞癌(舌癌)组织中Cks1、Skp2和p27kip1蛋白的表达,分析其与患者临床病理特征的关系,探讨其在舌癌的发生发展及预后中的作用。方法:采用免疫组织化学法检测45例舌癌组织、30例癌旁组织和10例正常舌黏膜组织中Cks1、Skp2和p27kip1蛋白的表达情况;比较不同类型舌组织中各蛋白表达阳性率的差异;分析Cks1、Skp2和p27kip1蛋白表达与舌癌临床病理学参数的关系。结果:在舌癌组织中,Cks1和Skp2蛋白呈强表达,阳性表达率为73.3%和62.2%,在癌旁组织和正常舌黏膜组织中其阳性表达率依次降低,阳性表达率分别为23.3%、10.0%和36.7%、10.0%;在舌癌组织中,p27kip1蛋白表达明显减少或缺失,阳性表达率为24.4%,在癌旁组织中和正常舌黏膜组织中其阳性表达水平依次升高,阳性表达率分别为30.0%和70.0%;在舌癌组织中Cks1和Skp2蛋白阳性表达率高于正常舌黏膜组织和癌旁组织(P<0.05),而p27kip1蛋白阳性表达率低于正常舌黏膜组织和癌旁组织(P<0.05)。舌癌组织中Cks1、Skp2和p27kip1蛋白阳性表达率与舌癌患者的年龄和性别无关联(P>0.05);与癌组织淋巴结转移、TNM分期、分化程度和临床分期有关联(P<0.05)。结论:舌癌组织中Cks1和Skp2蛋白阳性表达率升高、p27kip1蛋白阳性表达率降低,Cks1、Skp2和p27kip1蛋白阳性表达率的检测有助于判断舌癌的恶性程度和预后。Objective To detect the expressions of Cks1,Skp2,and p27kip1 proteins in tongue squamous cell carcinoma tissue and to analyze their associations with the clinicopathological characteristics of the patients with tongue squamous cell carcinoma,and to clarify their roles in the development and prognosis of tongue squamous cell carcinoma.Methods The expressions of Cks1,Skp2 and p27kip1 proteins in 45 cases of human tongue squamous cell carcinoma tissue,30 cases of adjacent carcinoma tissue and 10 cases of normal tongue mucosa tissue were detected by immunohistochemistry;the differences of the positive expression rates of Cks1,Skp2,and p27kip1 proteins in different kinds of tongue tissues were compared;the relationships between the expressions of Cks1,Skp2 and p27kip1 proteins with the clinicopathological parameters of the patients were analyzed.Results The expression levels of Cks1 and Skp2proteins in tongue carcinoma tissue were obviously increased and the positive expression rate of Cks1 and Skp2proteins were 73.3% and 62.2%.The expression levels of Cks1 and Skp2proteins in the adjacent carcinoma tissue and normal tongue mucosa tissue were decreased gradually,and the positive expression rates of Cks1 and Skp2proteins were 23.3%,10.0% and 36.7%,10.0%,respectively.The expression level of p27kip1 protein in the tongue carcinoma tissue was decreased or loss,and the positive expression rate of p27kip1 protein was 24.4%.The expression levels of p27kip1 proten in the adjacent carcinoma tissue and normal tongue mucosa tissue were increased gradually,and the positive expression rates of p27kip1 protein were30.0% and 70.0%,respectively.The postive expression rates of Cks1 and Skp2proteins in the tongue carcinoma tissue were higher than those in the adjacent carcinoma tissue and normal tongue mucosa tissue（P〈0.05）,and the positive expression rate of p27kip1 protein was lower than those in the adjacent carcinoma tissue and normal tongue mucosa tissue（P〈0.05）.The positive expression rates of Cks1,Skp2 and p27kip1 pr

关 键 词：周期素依赖性蛋白激酶亚单位1 S期激酶相关蛋白2 P27KIP1蛋白 舌鳞状细胞肿瘤 

分 类 号：R739.86[医药卫生—肿瘤]