DNMT3b基因多态性及其在胃癌组织中的表达水平与胃癌的关联性分析  

Associations between polymorphisms of DNMT3b gene and its expression level in gastric cancer tissue and gastric cancer

作　　者：王川[1] 贾志芳[1] 曹东慧[1] 武兴[1] 曹雪源[2] 姜晶[1] 

机构地区：[1]吉林大学第一医院临床流行病学研究中心,吉林长春130021 [2]吉林大学第一医院胃结直肠外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第2期369-373,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81072369;81273065;81373084)

摘　　要：目的:探讨中国北方汉族人群DNA甲基转移酶3b(DNMT3b)基因单核苷酸多态性(SNPs)和胃癌发病风险之间的关联性,阐明DNMT3b基因是否为胃癌的易感基因。方法:选择447例胃癌患者作为病例组,961名健康对照者作为对照组,采用TaqMan技术检测2组研究对象DNMT3b基因的rs1569686、rs6119954、rs4911107、rs4911259和rs8118663位点的基因型分布。免疫组织化学技术检测病例组(104例)胃癌患者胃癌组织和85例患者的癌旁对照组织中DNMT3b蛋白的表达水平。结果:在病例组和对照组研究对象中5个SNPs位点基因型分布频率相近,未发现5个SNPs位点的多态性与胃癌的发生有关联(P>0.05)。与癌旁组织比较,胃癌组织中DNMT3b蛋白的表达水平升高(P<0.05)。5个SNPs的不同基因型与胃癌组织中DNMT3b蛋白的表达水平均无关联(P>0.05)。结论:中国北方汉族人群中DNMT3b基因多态性与胃癌的发生无关联,DNMT3b基因可能不是胃癌的易感基因。Objective To investigate the associations between single nucleotide polymorphisms （SNPs） of DNA methyltransferase 3b （DNMT3b） gene and the risk of gastric cancer in the Han population from North China, and to clarify whether DNMT3b gene is the susceptible gene of gastric cancer. Methods 447 patients with gastric cancer were selected as case group and 961 healthy controls were used as control group. The distribution of genotypic frequencies of five SNPs （rs6119954, rs1569686, rs4911107, rs4911259, and rs8118663） in two groups were detected by TaqMan assay. The expression levels of DNMT3b protein in 104 cases of cancer tissue from the patients with gastric cancer and 85 cases of tumor-adjacent tissue from the patients with gastric cancer were analyzed by immunohistochemistry method. Results The distributions of genotypic frequencies of five SNPs in ease group and control group were similar. No significant association was found between the five SNPs and the risk of gastric cancer （P〉0.05）. Compared with the tumor-adjacent tissue, the expression level of DNMT3b protein in the gastric cancer tissue was increased （P〈0.05）. There were no siginificant differences between the genotypes ofthe five SNPs and the expression levels of DNMT3b protein （P〉0.05）. Conclusion There is no association between the polymorphisms of DNMT3b and the occurrence of gastric cancer in Han population from North China. DNMT3b gene may not be the susceptible gene of gastric cancer.

关 键 词：DNA甲基转移酶3B 胃肿瘤 单核苷酸多态性 

分 类 号：R735.2[医药卫生—肿瘤]