微波与超声-微波在秦岭龙胆挥发油分析中的对比研究  

Comparative Analysis of the Extraction of Volatile Oil from Gentiana apiata N. E. Br. by Means of MA and UME

作　　者：许海燕[1] 郑伶俐 多本加[3] 

机构地区：[1]陕西国际商贸学院医药学院,陕西咸阳712046 [2]步长集团咸阳制药厂,陕西咸阳712000 [3]青海师范大学民族学院,青海西宁810001

出　　处：《现代中药研究与实践》2015年第1期10-12,共3页Research and Practice on Chinese Medicines

基　　金：陕西省教育厅项目:秦岭龙胆有效部位的筛选与抗肝损伤作用的研究(2013JK0804)

摘　　要：目的研究秦岭龙胆用不同提取方法提取挥发油成分的异同。方法采用微波辅助提取法(MA)、超声-微波双辅助(UME)提取秦岭龙胆中的挥发油。采用GC-MS结合计算机检索对所得挥发油成分进行分析和鉴定,峰面积归一法测定各组分的相对含量。结果结果表明,2种方法分别从秦岭龙胆所得挥发油中鉴定出24及29种化合物。结论不同方法所得秦岭龙胆挥发油化学组成各有异同,但主要成分是2,2’-亚甲基双(四-甲基-6-叔丁基)-苯酚、棕榈酸、油酸、亚油酸等。Objective To study differences of chemical constituents between the two kinds of essential oils from Gentiana apiata N. E. Br. Methods Gentiana apiata N. E. Br. is a popular medicinal plant traditionally used in China to treat several diseases.In order to enlarge the medication scope,the constituents of essential oils which were extracted by means of microwave-assisted（MA） and ultrasonic-microwave synergistic extraction（UAMA） from Gentiana apiata N. E. Br. were analyzed and identifi ed with GC-MS. Results Based on the standard mass spectral data,24 and 29 kinds of volatile components were identifi ed respectively.Relative mass fractions of the constituents were determined by area normalization method.Conclusion There were differences in some chemical constituents among the two kinds of essential oils.But their main constituents were Oleic acid,Phenol, 2,2’-methylenebis[6-（1,1-dimethylethyl）, linoleic acid, palmitic acid, stearic acid and so on.

关 键 词：秦岭龙胆 挥发油 微波 超声-微波 化学成分 气相色谱-质谱联用 

分 类 号：R284.4[医药卫生—中药学]