海风藤提取物对胶原诱导性关节炎大鼠的影响  

Effects of Piperis kadsurae caulis extraction on rats with collagen-induced rheumatoid arthritis

作　　者：秦晴[1] 阙金花 张玉琴[1] 李煌[1] 徐伟[1] 褚克丹[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国民族民间医药》2015年第7期19-20,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金资助课题(81001624);校管课题(XB2011006)

摘　　要：目的:研究海风藤正丁醇提取物对胶原诱导性关节炎大鼠滑膜组织中NF-κB、IκB和IκK的影响,探讨其治疗作用机制。方法:通过建立胶原诱导性关节炎(CIA)大鼠模型,观察大鼠足趾肿胀度的变化,蛋白印迹法检测大鼠踝关节滑膜组织中NF-κB、IκB和IκK的表达水平。结果:海风藤正丁醇提取物能改善大鼠活动减少、精神不佳等一般状态,下调滑膜组织中NF-κB、IκK的表达及上调滑膜组织中IκB的表达。结论:海风藤正丁醇提取物对类风湿性关节炎有一定的治疗作用,其机理与调节滑膜组织中NF-κB、IκB和IκK的表达水平有关。Objective To investigate the therapeutic effect of Piperis kadsurae caulis extraction on collagen-induced rheumatoid arthritis rats,and its effects on NF-κB,IκB and IκK in synovial tissue,and to explore its therapeutic mechanism.Methods CIA rat model was induced by injecting bovine type II collagen.The swelling level of paw was observed and the expression of NF-κB,IKB and IκK were evaluated by Western blotting.Results The ethyl acetate extraces of Piperis kadsurae caulis could inhibit the paw swell-ing and down-regulate the expression of NF-κB,IκB and IκK.Conclusion The ethyl acetate extraces of Piperis kadsurae caulis have certain therapeutic on rheumatoid arthritis by reducing the expression of NF-κB,IκB and IκK in synovial tissue.

关 键 词：海风藤 胶原诱导性关节炎 NF-KB IΚB IκK 

分 类 号：R285.5[医药卫生—中药学]