半夏水提取液诱导人白血病K562细胞凋亡的初步研究  

Primary study on apoptosis of K562cells in vitro induced by water extract of Pinellia ternate(Thunb)Breit

作　　者：邬萌[1] 陈斌[2] 

机构地区：[1]湖北中医药大学,武汉420061 [2]湖北省中医院

出　　处：《临床血液学杂志》2014年第6期983-986,共4页Journal of Clinical Hematology

摘　　要：目的:观察半夏水提取液对K562细胞增殖及凋亡的影响,初步探讨半夏对白血病的抗肿瘤作用机制。方法:采用CCK-8法,检测半夏水提取液不同浓度梯度范围内(35、350、700、1 000μg/ml)及不同作用时间(24、48、72h)对K562细胞的增殖抑制率;流式细胞仪检测K562细胞48h凋亡及周期变化。结果:半夏水提取液对人白血病K562细胞的增殖有明显的抑制作用,且呈剂量依赖性和时间依赖性;流式细胞仪分析结果显示半夏水提取液作用K562细胞48h后细胞凋亡百分率随半夏水提取液浓度增加而增加,且半夏水提取液可以诱导K562细胞周期阻滞在G0/G1期。结论:半夏水提取液在一定浓度范围内对人白血病细胞K562的增殖有抑制作用,且呈一定的量效时效关系;其对K562细胞的抑制作用可能与其诱导细胞凋亡和细胞周期阻滞有关。Objective：To observe the inhibition of proliferation and the apoptosis induced by water extract of Pinellia ternate （Thunb） Breit in leukemia K562 cells,and to discuss the antitumor mechanism of leukemia. Meth- od：After treated with water extract of Pinellia ternate（Thunb） Breit at different concentration gradient （35,350, 700,1 000 μg/ml） and at different times （24,48,72 h） ,the inhibition rate of the proliferation of K562 cells was de- tected by CCK-8 assay. The activity of shikonin in inducing apoptosis and the distribution of cell cycle after 48 h were investigated by flow cytometry. Result：Water extract of Pinellia ternate （Thunb） Breit could inhibit the pro- liferation of K562 cells,in a dose and time dependent manner. The apoptosis percentage detected by flow cytometry increased as the water extract of Pinellia ternate （Thunb） Breit concentration increased. And it could arrest K562 in G0/G1 phase. Conclusion：Water extract of Pinellia ternate （Thunb） Breit at concentration ranging from 35 to 1 000 μg/ml could inhibit the proliferation of K562 cells in a time and dose dependent manner, which may be due to induction of apoptosis and cell cycle arrest.

关 键 词：半夏 K562细胞 细胞增殖 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]