重庆地区马铃薯上黄瓜花叶病毒的检测及亚组鉴定  

Detection and Subgrouping of Cucumber Mosaic Virus(CMV) Infecting Potato Plants in Chongqing

作　　者：吴畏[1] 孙淼[1] 孙现超[1] 周常勇[1,2] 青玲[1,2] 

机构地区：[1]西南大学植物保护学院植物病害生物学重庆市高校级重点实验室,重庆400716 [2]中国农业科学院柑橘研究所,重庆400712

出　　处：《西南大学学报（自然科学版）》2014年第7期29-32,共4页Journal of Southwest University(Natural Science Edition)

基　　金：农业部公益性行业科研专项资助项目(201303028);国家自然科学基金资助项目(31272013)

摘　　要：采用抗原直接包被ELISA对采自重庆11个马铃薯主产区的259份马铃薯病毒病样品进行了黄瓜花叶病毒Cucumber mosaic virus,CMV的检测及亚组鉴定.共有233份样品检测到CMV,总检出率达91.60%;其中属CMVⅠ亚组的样品为197份,占84.55%;另36份样品为CMVⅡ亚组侵染,占15.45%;未检测到CMVⅠ和CMVⅡ的复合侵染.这些结果表明,CMVⅠ是重庆地区马铃薯上的优势CMV亚组类型.Two hundred and fifty nine potato leaf samples with viral symptoms were collected from eleven potato growing counties in Chongqing and were tested for detecting Cucumber mosaic virus （CMV） and its subgroups by using direct antigen coated enzyme linked immunosorbent assay （ELISA）. Two hundred and thirty-three samples were shown to be infected by CMV, which was 91.60% of the total samples. Of the CMV-positive sa group II （CMV mp II） es, 84.55% were infected by CMV subgroup I （CMV I） and 15.45 No co-infection of CMV I and CMV II was detected. These resu CMV I was the dominant subgroup of CMV infecting potato crops in Chongqing, China

关 键 词：马铃薯 黄瓜花叶病毒 检测 亚组 

分 类 号：S632[农业科学—蔬菜学]