莫诺苷对局灶性脑缺血-再灌注大鼠海马CyclinD1及CDK6的影响  

Effects of Morroniside on the Expression of CyclinD1 and CDK6 in Hippocampus of Rats Treated with Focal Cerebral Ischemia/Reperfusion Injury

作　　者：刘婷婷[1] 孙芳玲[1] 艾厚喜[1] 张丽[1] 王文[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室,北京市老年病医疗研究中心

出　　处：《神经药理学报》2013年第4期1-7,共7页Acta Neuropharmacologica

基　　金："重大新药创制"科技重大专项项目(No.2012ZX09102-201-016);国家自然科学基金项目(No.81173575);北京市自然科学基金项目(No.7062031);北京市教委科技创新平台项目(No.111219);2011年北京市卫生系统高层次卫生技术人才培养计划项目(No.2011-3-097);第52批中国博士后基金项目(No.2012M520330);北京市博士后科研活动经费资助项目(No.106455)

摘　　要：目的:观察莫诺苷对局灶性脑缺血-再灌注大鼠细胞周期蛋白Cyclin D1及CDK6的影响。方法:用线栓法制备大鼠局灶性脑缺血-再灌注模型。将15只Sprague-Dawley(SD)大鼠随机分为假手术组,模型组,莫诺苷组(270、90、30 mg·kg-1)。利用免疫组化方法检测大鼠患侧海马Cyclin D1、CDK6表达。结果:与假手术组相比,模型组Cyclin D1及CDK6表达显著增加;给予莫诺苷治疗后,与模型组相比,莫诺苷(270、90 mg·kg-1)剂量组能显著降低大鼠Cyclin D1及CDK6的表达。结论:莫诺苷能通过降低脑缺血-再灌注后Cyclin D1及CDK6的表达而发挥神经保护作用。Objective： To explore the effects of morroniside on key cell-cycle regulator proteins Cyclin D1 and CDK6 in a rat model of focal cerebral ischemia-reperfusion（I-R）. Methods： After the model of middle cerebral artery occlusion（MCAO） was established,15 Sprague-Dawley（SD） rats were randomly assigned into sham group,model group,morroniside group（270、90、30 mg·kg-1）. Immunohistochemistry staining was used to measure the expression of Cyclin D1 and CDK6 in ischemic ipsilateral hippocampus. Results： Compared with sham group,the expression of Cyclin D1 and CDK6 were signifi cantly increased in vehicle-treated I-R rats. However,the expression of Cyclin D1 and CDK6 was signifi cantly reduced by treatment of morroniside at doses of 270 mg·kg-1and 90 mg·kg-1 after I-R compared with control group. Conclusion： Morroniside might play a role of neuroprotection by down-regulating the expression of Cyclin D1 and CDK6 after cerebral I-R treatment.

关 键 词：莫诺苷 脑缺血-再灌注 细胞周期蛋白 神经保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学]