宫颈鳞状细胞癌EGFR、c-jun和c-fos表达与临床特征及近期疗效的相关性  

Correlation of expressions of EGFR, c-jun, and c-fos of cervical squamous cell carcinomas and clinical features and short-term efficacy

作　　者：赵俊玲[1] 马栋辉[1] 古丽娜.库尔班 王若峥[1] 

机构地区：[1]新疆医科大学附属肿瘤医院放疗中心,乌鲁木齐830011

出　　处：《上海交通大学学报（医学版）》2015年第2期216-222,232,共8页Journal of Shanghai Jiao tong University:Medical Science

基　　金：科技部国际合作项目(2012DFA31560)~~

摘　　要：目的探讨宫颈鳞状细胞癌(CSCC)表皮生长因子受体(EGFR)、原癌基因c-jun、c-fos表达与临床特征及近期疗效的相关性。方法荧光原位杂交(FISH)技术及免疫组织化学法检测60例CSCC组织EGFR基因拷贝数及EGFR、c-jun、c-fos蛋白表达水平。结果 EGFR基因拷贝数:二倍体32.2%、三倍体25.4%、多倍体30.5%、基因扩增率11.9%;EGFR、c-jun和c-fos蛋白阳性表达率分别为70.0%、76.7%和68.3%,三者均与肿瘤分化程度、淋巴结转移有关(P均<0.01);EGFR蛋白表达与临床分期有关(P<0.01);Spearman等级相关分析显示:EGFR蛋白表达水平与基因拷贝数增加呈正相关(r=0.622,P=0.000),c-jun和c-fos蛋白表达与临床分期呈正相关(r=0.293,P=0.023和r=0.296,P=0.022);EGFR与c-jun、c-fos蛋白表达水平呈正相关(r=0.422,P=0.001;r=0.509,P=0.000);EGFR、c-jun和c-fos蛋白表达对客观有效率无明显影响(P>0.05)。结论 EGFR、c-jun和c-fos表达可能为CSCC高危进展、转移的参考指标;三者可能均不是影响CSCC近期疗效的独立因素;c-jun和c-fos在EGFR介导的信号转导通路中可能发挥正性调节作用。Objective To investigate the correlation of expressions of epidermal growth factor receptor（ EGFR） and proto-oncogenes c-jun and c-fos of cervical squamous cell carcinomas（ CSCC） and clinical features and short-term efficacy. Methods The fluorescence in situ hybridization（ FISH） and immunohistochemistry method were adopted to detect the number of EGFR gene copies and protein expression levels of EGFR, c-jun, and c-fos of CSCC tissues of 60 cases. Results The numbers of EGFR gene copies were 32. 2% for diploid, 25. 4% for triploid, 30. 5% for polyploid, and 11. 9% for gene amplification. The positive protein expression rates of EGFR, c-jun, and c-fos were70. 0%, 76. 7%, and 68. 3% and correlated with the lymph node metastasis and tumor differentiation（ P〈0. 01）. The protein expression of EGFR correlated with the clinical stages（ P〈0. 01）. Spearman rank correlation analysis showed that the protein expression level of EGFR positively correlated with the increase of gene copies（ r = 0. 622, P =0. 000）; protein expressions of c-jun and c-fos positively correlated with the clinical stages（ r = 0. 293, P = 0. 023; r= 0. 296, P = 0. 022）; the protein expression level of EGFR positively correlated with protein expressions levels of cjun and c-fos（ r = 0. 422, P = 0. 001; r = 0. 509, P = 0. 000）; and protein expressions of EGFR, c-jun, and c-fos did not significantly affect the objective efficiency（ P〉0. 05）. Conclusion Expressions of EGFR, c-jun, and c-fos may be reference indexes of the development and metastasis of CSCC and may not be independent factors that affect the short-term efficacy of CSCC. The c-jun and c-fos may play a positive regulatory role in EGFR-mediated signal transduction pathway.

关 键 词：宫颈鳞状细胞癌 表皮生长因子受体 C-JUN C-FOS 近期疗效 

分 类 号：R737.33[医药卫生—肿瘤]