三氧化二砷对肾母细胞瘤增殖及凋亡的影响  

Effects of As_2O_3 on proliferation and apoptosis of SK-NEP-1 cells of human Wilms tumor

作　　者：卢立慧 薛天阳[1] 许伟[1] 高吉照[1] 

机构地区：[1]徐州医学院附属医院儿科,徐州221000

出　　处：《上海交通大学学报（医学版）》2015年第2期291-294,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的观察三氧化二砷(As2O3)对体外人肾母细胞瘤SK-NEP-1细胞株增殖和凋亡的影响。方法应用0、1.25、2.5、5、10μmol/L As2O3(A、B、C、D、E组)作用SK-NEP-1细胞24 h。采用MTT比色法检测不同浓度As2O3对SK-NEP-1细胞的增殖抑制率;流式细胞仪检测凋亡率;ELISA法检测SK-NEP-1细胞Bcl-2水平变化;Caspase 3试剂盒检测细胞Caspase 3活性。结果 A、B、C、D、E组细胞增殖抑制率分别为(3.25±1.05)%、(11.19±2.27)%、(20.72±2.21)%、(27.24±0.57)%、(32.25±2.15)%,细胞凋亡率分别为(5.12±0.29)%、(13.42±1.03)%、(23.70±0.97)%、(32.44±0.82)%、(40.22±1.31)%,各组间差异均具有统计学意义(均P<0.05)。B、C、D、E组Bcl-2蛋白表达水平均低于A组(均P<0.05),Caspase 3活性均高于A组(均P<0.05)。结论 As2O3能明显抑制肾母细胞瘤细胞的增殖,并进一步诱导其凋亡,其抗肿瘤机制可能与下调Bcl-2表达及激活Caspase 3有关。Objective To investigate the effects of As2O3 on the proliferation and apoptosis of SK-NEP-1 cells of human Wilms tumor in vitro. Methods The SK-NEP-1 cells of human Wilms tumor was treated with As2O3 of 0, 1. 25, 2. 5, 5, and 10 μmol / L（ group A, B, C, D, and E） for 24 h. The proliferation inhibition rates of different concentrations of As2O3 towards SK-NEP-1 cells were detected by the MTT assay, the apoptosis rates were detected by the flow cytometry; variations of the Bcl-2 level of SK-NEP-1 cells were detected by the ELISA; and the Caspase 3 activity was detected by the Caspase 3 Kit. Results The proliferation inhibition rates of A, B, C, D, and E groups were（ 3. 25 ± 1. 05） %,（ 11. 19 ± 2. 27） %,（ 20. 72 ± 2. 21） %,（ 27. 24 ± 0. 57） %, and（ 32. 25 ± 2. 15） %, respectively. The apoptosis rates of 5 groups were（ 5. 12 ±0. 29） %,（ 13. 42 ± 1. 03） %,（ 23. 70 ± 0. 97） %,（ 32. 44 ± 0. 82） %, and（ 40. 22 ± 1. 31） %,respectively. The differences of 5 groups were statistically significant（ P〈0. 05）. The expression level of Bcl-2 of B, C, D, and E groups was lower than that of the A group（ P〈0. 05） and the Caspase 3 activity was higher than that of the A group（ P〈0. 05）. Conclusion As2O3 can significantly inhibit the proliferation of SK-NEP-1 cells and induce the apoptosis. The mechanism may be relevant to down-regulating the expression of Bcl-2 and increasing the Caspase 3 activity.

关 键 词：三氧化二砷 肾母细胞瘤 细胞凋亡 

分 类 号：R737.11[医药卫生—肿瘤]