根癌农杆菌介导的地衣型真菌Cladonia metacorallifera的转化  

Agrobacterium tumefaciens-mediated transformation of the lichen forming fungus Cladonia metacorallifera

作　　者：王毅[1,2] 王晨晨[1,3] 周旭[1,3] 许宰铣 王娟[2] 

机构地区：[1]云南林业科学院国家林业局云南珍稀濒特森林植物繁育和保护重点实验室,云南昆明650201 [2]云南林业科学院云南省森林植物培育与开发利用重点实验室,云南昆明650201 [3]西南林业大学林学院,云南昆明650224 [4]韩国国立顺天大学韩国地衣研究所,韩国顺天540742

出　　处：《菌物学报》2015年第2期246-251,共6页Mycosystema

基　　金：国家自然科学青年科学基金项目(31400488);云南省中青年学术技术带头人后备人才培养项目(2010CI016)

摘　　要：以潮霉素抗性和增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)作为筛选标记,利用地衣型真菌Cladonia metacorallifera的菌丝,成功实现了根癌农杆菌介导的遗传转化,PCR检测证明转化子中存在潮霉素抗性基因,共聚焦显微镜检测到转化子菌丝能够产生绿色荧光,证明EGFP能够在trp C启动子控制下在地衣型真菌中表达。Agrobacterium tumefaciens-mediated transformation （ATMT） was successfully applied to Cladonia metacorallifera. Vegetative mycelium of this fungus was used for transformation by ATMT. resistance （hph） and the enhanced green fluorescent protein （EGFP as selection markers. PCR amplification of hph and the observation metacorallifera was successfully transformed by ATMT. the lichen forming fungus The genes for hygromycin ）, both under the control of a trpC promoter, were employed of fluorescence signal by confocal microscopy showed that C.

关 键 词：CLADONIA metacorallifera 农杆菌介导的转化 增强型绿色荧光蛋白 潮霉素抗性 

分 类 号：Q949.3[生物学—植物学]