碳青霉烯酶药物不敏感的肠杆科细菌中KPC酶的检测及分析  

KPC carbapenemases among Enterobacteriaceae with carbapenem non-susceptibility

作　　者：黄会[1,2] 佘姝敏 詹希美[4] 吴多荣[1,2] 

机构地区：[1]海口市人民医院 [2]中南大学湘雅医学院附属海口医院检验科,海口570208 [3]海南医学院热带医学与检验医学院,海口570208 [4]中山大学中山医学院病原生物部,广州510080

出　　处：《中国人兽共患病学报》2015年第3期247-250,共4页Chinese Journal of Zoonoses

基　　金：海口市重点科技计划项目(No.2012-070)~~

摘　　要：目的了解海南地区三级医院中肠杆菌科细菌产KPC酶的流行状况及型别特点。方法收集2012年8月到2013年6月海南地区四家三级医院无菌部位分离的对亚胺培南或厄他培南中介或耐药的肠杆菌科细菌43株,运用改良Hodge试验进行KPC表型筛查;PCR的方法进行基因型别检测。结果 43株细菌中,21株改良Hodge试验阳性;PCR检测3株为阳性,基因测序比对分析均为KPC-2型。结论海南地区对碳青霉烯酶类药物不敏感的肠杆科细菌中KPC酶的主要基因型别KPC-2型,携带KPC酶的质粒可在不同种属细菌间水平传递,临床应加强监控,防止产碳青霉烯酶菌株在医院环境中暴发和流行。To investigate the prevalence and gene types of KPC in Enterobacteriaceae strains isolated from 4 tertiary general hospitals in Hainan area ,a total 43 isolates which were resistant or intermediate to imipenem or ertapenem were collected from sterile sites between August 2012 and June 2013 from 4 tertiary general hospitals in Hainan area .Modified Hodge Tests （M HT ） were performed for KPC phenotype screening .PCR amplification and DNA sequence were performed to analyze the encoding genes of KPC .Results showed that in the 43 isolates ,21 strains were positive in M HT .PCR and DNA sequence analysis confirmed that 3 isolates produced KPC‐2 .It＆#39;s suggested that there were the Enterobacteriaceae carrying KPC in Hain‐an area .The encoding genes were KPC‐2 .The KPC gene could be horizontally transmitted by plasmid among different groups of bacteria .It is important to control the transmission of these Enterobacteriaceae carrying KPC .

关 键 词：碳青霉烯酶 KPC 肠科杆细菌 改良HODGE试验 

分 类 号：R378.2[医药卫生—病原生物学]