结直肠癌组织中血管抑制蛋白1表达的临床意义及其与血管内皮生长因子A和微血管密度的相关性  

Expression of vasohibin-1 in colorectal cancer tissue and its correlation with vascular endothelial growth factor A and microvessei density

作　　者：史刚刚[1] 郝敬鹏[1] 王力[1] 何彬[1] 甘建琛[1] 王晖[1] 所荣增[1] 

机构地区：[1]天津医科大学第二医院肛肠外科,300211

出　　处：《中华胃肠外科杂志》2015年第3期272-276,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金青年项目（81302211）;天津市卫生局科技基金项目（2013KZ107）

摘　　要：目的：探讨结直肠癌组织中血管抑制蛋白1（Vasohibin-1）表达的临床意义及其与血管内皮生长因子A（VEGF-A）和微血管密度（MVD）的相关性。方法收集天津医科大学第二医院2013年6月至2013年12月手术切除并经病理证实的60例结直肠癌及癌旁（距肿瘤大于5 cm）组织标本，应用免疫组织化学染色法检测上述组织中Vasohibin-1和VEGF-A的表达情况及MVD （CD34标记），并分析三者的相关性；应用反转录PCR和Western-blot检测Vasohibin-1和VEGF-A mRNA及蛋白的表达情况；分析Vasohibin-1和VEGF-A表达与结直肠癌患者临床病理因素的关系。结果 Vasohibin-1主要表达于癌细胞及血管内皮细胞质中，VEGF-A主要表达于癌细胞的胞质和胞膜。结直肠癌组织标本中Vasohibin-1与VEGF-A的阳性表达率、蛋白表达量及mRNA水平均明显高于癌旁组织（均P＜0．05）。 Vasohibin-1表达与VEGF-A表达（r＝0．378，P＝0．003）及MVD（r＝0．628，P＜0．001）呈正相关。 Vasohibin-1阳性表达率与结直肠癌的TNM分期和远处转移有关，分期越晚及有远处转移者阳性表达率越高（均P＜0．05）。结论结直肠癌组织中Vasohibin-1的表达与VEGF-A表达和MVD呈正相关。 Vasohibin-1的阳性表达提示结直肠癌患者预后不良。Objective To explore the clinical significance of vasohibin-1 expression in colorectal cancer tissues and its correlation with vascular endothelial growth factor A （ VEGF-A ） and microvessel density （MVD）. Methods Tumor tissues and paired adjacent normal tissue （distance to cancer 〉5 cm） from 60 colorectal cancer patients undergoing resection in the Second Hospital of Tianjin Medical University from June 2013 to November 2013 were included in this study. The protein expressions of vasohibin-1, VEGF-A and MVD were detected by immunohistochemical staining. The mRNA expressions of vasohibin-1 and VEGF-A were detected by RT-PCR. The protein expressions of vasohibin-1 and VEGF-A were observed by Western blot. Correlation among parameters was examined. Results Vasohibin-1 expression was mainly localized in the cytoplasm of tumor cells and endothelial cells. VEGF-A expression was mainly localized in the cytoplasm and membrane of tumor cells. The expressions of vasohibin-1, VEGF-A and MVD in colorectal tumor tissues were significantly higher than those in corresponding adjacent tissues [43.3%（26/60） vs. 16.7%（10/60）, 51.7%（31/60） vs. 18.3%（11/60）, （39.67±16.80）/mm2 vs. （17.85±6.43）/mm2, all P〈0.05]. Higher vasohibin-1 expression was significantly associated with TNM stage and metastasis （P〈0.05）. Vasohibin-1 expression was positively correlated with VEGF-A and MVD （r=0.378, 0.628, all P〈0.05）. Vasohibin-1 and VEGF-A mRNA expressions and protein expressions in colorectal cancer tissues were significantly higher than those in corresponding adjacent tissues （all P〈0.05）. Conclusion Vasohibin-1 expression in colorectal cancer tissues is significantly higher as compared to corresponding adjacent tissues. Vasohibin-1 expression is positively correlated to VEGF-A and MVD, and associated to TNM stage and metastasis. Positive vasohibin-1 expression indicates a poor prognosis of patients with colorectal cancer.

关 键 词：结直肠肿瘤 血管抑制蛋白1 血管内皮生长因子A 微血管密度 

分 类 号：R735.34[医药卫生—肿瘤]