Characterization of four hemocyanin isoforms in Litopenaeus vannamei  

作　　者：XU Jingxiang RUAN Lingwei LI Zhen YU Xiaoman LI Sedong SHI Hong XU Xun 

机构地区：[1]School of Life Science, Xiamen University [2]Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography, State Oceanic Administration [3]Zhanjiang Evergreen South Ocean Science & Technology Company Limited [4]School of Life Sciences, Sun Yat-Sen University [5]Zhanjiang Ocean and Fishery Development Research Center

出　　处：《Acta Oceanologica Sinica》2015年第2期36-44,共9页海洋学报（英文版）

基　　金：The National High Technology Research and Development Program(863 Program)of China under contract No.2012AA092205;the Major State Basic Research Development Program(973 Program)of China under contract No.2012CB114403;the China Agriculture Research System-47;the National Natural Science Foundation of China under contract No.31472297 and 31302209;the Scientific Research Foundation of Third Institute of Oceanography,SOA under contract No.2011018

摘　　要：In this study, the gene encoding hemocyanin subunit L, Lv Hc L, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them（Lv Hc L4） only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of Lv Hc L were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in invertebrate.In this study, the gene encoding hemocyanin subunit L, Lv Hc L, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them（Lv Hc L4） only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of Lv Hc L were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in invertebrate.

关 键 词：HEMOCYANIN SNPS ISOFORMS Litopenaeus vannamei WSSV 

分 类 号：S917.4[农业科学—水产科学]