酵母常规透射电镜样品制备方法的比较与改进  

Comparison and optimization of the conventional methods for yeast sample preparation for transmission electron microscopy

作　　者：梁静南[1] 杨琳[2] 赵彤[1] 刘一苇[1] 

机构地区：[1]中国科学院微生物研究所,北京100101 [2]中国科学院遗传与发育生物学研究所分子发育生物学国家重点实验室,北京100101

出　　处：《电子显微学报》2015年第1期85-88,共4页Journal of Chinese Electron Microscopy Society

摘　　要：透射电镜技术中前期样品制备是获得最接近样品真实结构信息的关键。本文选择酵母为观察对象,运用常规样品制备技术,观察不同缓冲液、不同脱水时间和脱水温度对酵母超微结构的影响,优化了酵母常规透射电镜前期制样方案。Sample preparation for transmission electron microscopy is the most important step to preserve the biological ultrastructure. In this study, yeast cells were treated with different buffers, and dehydrated in different time periods at different temperatures. By morphological comparison, we got an optimized strategy for conventional method for yeast cell sample preparation for transmission electron microscopy.

关 键 词：酵母 透射电镜 缓冲液 脱水 

分 类 号：Q-336[生物学]