植物酯酶源的筛选及最佳提取条件的确定  

Screen of Plant Esterase and Optimum Extraction Condition

作　　者：张天灵[1] 魏金会[1] 李洋[1] 于海生[1] 王亚飞[1] 

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319

出　　处：《黑龙江农业科学》2015年第4期133-137,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江八一农垦大学校级大学生创新实验资助项目

摘　　要：为建立蔬菜、水果中农药残留的快速检测方法,以2,6-二氯乙酰靛酚为显色剂,对不同来源的植物酯酶进行筛选,以总酯酶活力和比活力为考核指标,确定最佳植物酯酶源。结果表明:苜蓿酯酶的活力和比活力最大,苜蓿为最佳植物酯酶源,酯酶最佳提取条件是在4℃,以pH7.0的0.1mol·L-1磷酸盐缓冲溶液为提取剂,料液比1∶10,提取时间10min。In order to set up a rapid detection for the pesticide residue in vegetables and fruits,taking 2,6-dichloro-acetyl indophenol as chromogenic agent,different sources of plant esterases were contrasted in the presence of pesticide.The results showed that the total activity and the specific activity of the alfalfa esterase were the highest compared with other sources,so the optimum source was from alfalfa.The optimum conditions as follows：the temperature below 4℃;pH 7.0;0.1mol·L-1 phosphate buffered solution as the extracting agent;soild-liquid ratio 1∶10;and the extraction time for 10 min.

关 键 词：植物酯酶 筛选 提取条件 

分 类 号：S481.8[农业科学—农药学]