利多卡因对肠缺血-再灌注幼鼠肺保护作用的观察  

Protective effect of lidocaine on lung injury induced by intestinal ischemia-reperfusion in young rats

作　　者：曲文超[1] 林春榕[2] 吴学东[1] 潘云[1] 黄勇[1] 李娜[1] 

机构地区：[1]大理学院附属医院暨临床医学研究中心,云南大理671000 [2]大理学院基础医学院生物化学与分子生物学教研室,云南大理671000

出　　处：《中国现代医学杂志》2015年第9期41-46,共6页China Journal of Modern Medicine

基　　金：云南省自然科学基金(No:2009ZC121M)

摘　　要：目的探讨利多卡因对幼鼠肠缺血-再灌注(II/R)所致肺脏损伤的保护作用。方法 4周龄SD大鼠80只随机分为四组:假手术组(Sham组)和缺血组(II组)各10只,缺血-再灌注组(II/R组)和利多卡因干预组(Lid组)各30只。Sham组仅暴露腹腔脏器,不作任何干预;II组为阻断肠系膜上动脉(SMA)1 h;II/R组经阻断SMA 1 h后恢复血供,再灌注2 h,建立II/R损伤模型;Lid组于再灌注前10 min经股静脉注射利多卡因2 mg/kg,II/R组注射等剂量的生理盐水。于再灌注0.5、1和2 h进行肺组织H-E染色并研究各组肺组织丙二醛(MDA)、髓过氧化物酶(MPO)水平及细胞间黏附分子1(ICAM-1)的表达,检测结果的组间差异进行统计学分析。结果肠缺血1 h即出现肺组织损伤,再灌注后肺损伤进行性加重,利多卡因干预后肺组织损伤有所减轻;肺组织中ICAM-1、MPO和MDA水平检测结果在组间比较,II组和Sham组间各指标测得值的差异均无统计学意义(P>0.05),II/R组肺组织中各指标均明显高于其他三组,差异有统计学意义(P<0.01);Lid组中MPO活性仍高于Sham组(P<0.01)和II组(P<0.05),MDA水平仅高于Sham组(P<0.05),ICAM-1的表达也仅在再灌注1 h时高于Sham组(P<0.01),Lid组其余时间点MDA和ICAM-1测得值均未高于Sham组(P>0.05)。结论 II/R导致幼鼠肺组织损伤并随再灌注时间延长而加重,利多卡因减轻了再灌注阶段产生的有害因子对肺组织的损伤。【Objective】To investigate the protective effect of lidocaine on lung injury induced by intestinal ischemia-reperfusion（II/R） in young rats.【Methods】A total of 80 Sprague Dawley（SD） rats at 4 weeks of age were divided into 4 groups in random, which were sham operation group（Sham group, n = 10） as a control with no intervention after abdominal opening, intestinal ischemia group（group II, n = 10） undergoing superior mesenteric artery（SMA） occlusion for 1 h, and intestinal ischemia-reperfusion group（group II/R, n = 30） with blood re-supply for 2 h after SMA occlusion for 1 h, lidocaine group（group Lid, n = 30） with injection of lidocaine 2 mg/kg via femoral vein10 min before reperfusion and equal volume of saline was injected in group II/R as control. Histological study of lung morphology, malondialdehyde（MDA）, myeloperoxidase（MPO） and intercellular adhesion molecule（ICAM-1） in the lung tissue were investigated at 0.5, 1 and 2 h following reperfusion and 10 rats were harvested at each time point. The data were statistically analyzed. 【Results】Morphological changes of the lung tissue injury were observed at 1 h after intestinal ischemia and then progression extended with time of reperfusion, but the damage reduced somewhat after lidocaine intervention. The values of ICAM-1, MPO and MDA in the group II/R were higher than those in the remaining three groups with statistical significance（P 〈 0.01）, but there were no significant differences between the Sham group and the group II（P 〉 0.05）. In the group Lid, the value of MPO was higher than that in the Sham group（P 〈 0.01） and the group II（P 〈 0.05）; the MDA level and ICAM-1 expression at 1 h after reperfusion were significantly higher than those in the Sham group（P 〈 0.05 and P 〈 0.01, respectively）, but not at other time points（P 〉 0.05）.【Conclusions】Intestinal ischemia-reperfusion can induce lung injury in young rats which is aggravating with prolonged reperfusion time, and th

关 键 词：肠缺血-再灌注肺损伤 利多卡因 保护作用 幼鼠 

分 类 号：R-332[医药卫生]