荞麦中芦丁对免疫性肝损伤小鼠肝脏超微结构的影响  

Effect of Buckwheat rutin on the liver ultrastructure in immunological hepatic injury mice

作　　者：储金秀[1] 李倩[2] 李光民[3] 张博男[1] 勾向博[1] 韩淑英[1] 

机构地区：[1]河北联合大学基础医学院唐山市慢性病临床基础研究重点实验室,河北唐山063000 [2]河北联合大学医学实验研究中心,河北唐山063000 [3]唐山市妇幼保健院放射科,河北唐山063000

出　　处：《华西药学杂志》2015年第2期190-192,共3页West China Journal of Pharmaceutical Sciences

基　　金：河北省自然基金项目(C2010001795);河北省教育厅青年基金项目(2011156)

摘　　要：目的观察荞麦中芦丁(RBFL)对卡介苗(BCG)和脂多糖(LPS)联合诱导的免疫性肝损伤小鼠肝脏的超微结构的影响。方法于尾静脉注射BCG和LPS,建立小鼠免疫性肝损伤模型。取♂昆明小鼠90只,随机均分为正常组、模型组、RBFL低、中、高剂量组及联苯双酯组。除正常组和模型组ig蒸馏水外,其余各组均ig给予对应药物干预,即40、80、160 mg·kg-1·d-1RBFL及联苯双酯200 mg·kg-1·d-1,gd,连续12 d。剖取肝脏、脾脏、胸腺,计算脏器指数,电镜下观察各组小鼠的肝脏超微结构变化。结果模型组小鼠的肝脏体积增大,肝脏指数较正常组的显著增大(P<0.01),各免疫器官脏器的指数增大,RBFL各剂量有抑制作用。结论 RBFL对BCG和LPS联合诱导的小鼠肝脏超微结构损伤具有保护作用。OBJECTIVE To observe the effects of the rutin from Buckwheat flowers and leaves （RBFL） on liver uhrastructures in immunologica/hepatic injury mice induced by BCG and LPS. METHODS Immune hepatic injury model of mice was induced by injecting BCG and LPS from the caudal vein. Ninety male Kunming mice were randomly divided into normal control group, model group, RBFL- L group,RBFL- M group,RBFL- H group and positive control drug （Bifendate） group. The rats were daily gastrically injected with different concentrations of RBFL（40,80,160 mg·kg^-1·d^-1 ） and Bifendate （200 mg·kg^-1 ） for 12 d. Distilled water of the same volume was given to the mice in normal control group and model group. Immune organs including the liver, spleen and thymus were weighed and the organ indexes were calculated. The livers of all the mice were taken to observe the ultrastructures with electron micro- scope. RESULTS The livers of model group appeared much larger than the normal ones, the LI was increased remarkably and the immune organ indexes were increased. RBFL could inhibit the elevation of immune organ indexes induced by LPS and BCG. CONCLU- SION RBFL has protective effect on liver ultrastructures in the immunological hepatic injury mice induced by BCG and LPS.

关 键 词：荞麦 芦丁 肝损伤 超微结构 卡介苗 脂多糖 联苯双酯 脏器指数 

分 类 号：R96[医药卫生—药理学]