Silica sol/Nano-Au/PVA/L-cysteine修饰金电极电致化学发光测定甲氧氯普胺的研究  

Study on electrochemiluminescence determination of metoclopramide on silica sol / Nano-Au / PVA / L-cysteine modified gold electrode

作　　者：李彦青[1] 罗应[2] 李利军[1] 程昊[1] 黄文艺[1] 

机构地区：[1]广西科技大学生物与化学工程学院,柳州545006 [2]广西科技大学鹿山学院,柳州545006

出　　处：《分析试验室》2015年第4期373-378,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(20665001);广西科学基金资助项目(0640029);广西高校科学技术研究项目(2013YB176)资助

摘　　要：在甲氧氯普胺对联吡啶钌具有较好电化学发光增敏效果的基础上,制备出Silica sol/Nano-Au/PVA/L-cysteine修饰金电极,并通过电致化学发光法,考察了联吡啶钌及联吡啶钌-甲氧氯普胺体系在此电极上的电化学及电化学发光行为。该修饰电极对联吡啶钌-甲氧氯普胺体系有良好的电致化学发光响应特性;在最优条件下,在1.0×10-7～1.0×10-4mol/L范围内甲氧氯普胺浓度与其对应的电化学发光强度值线性关系良好,检出限（S/N=3）为1.40×10-9mol/L;通过平行测定1.0×10-5mol/L甲氧氯普胺溶液8次,发光强度值相对偏差（RSD）为1.8%,样品回收率在98.3%～104.4%之间,RSD为2.3%（n=5）。An electrochemiluminescence （ECL） method was developed for the determination of metoclopramide based on the sensitizing effect of metoclopramide on Ru （bpy）3^2＋, and a silica sol/nano-Au/PVA/L-cysteine modified gold electrode was prepared for the simple, sensitive and accurate determination of metoclopramide, moreover, the electrochemical behaviors and the electrochemical luminescence behaviors of the Ru （bpy）3^2＋system and the Ru（bpy） 3^2＋ -metoclopramide system were investigated by ECL respectively. The results suggest that the proposed electrode shows very good electrochemical activity and electroehemiluminescence response to the Ru （bpy）3^2＋-metoclopramide system. Under the optimum experimental conditions, the linearity of metoclopramide concentration to ECL intensity was achieved in the concentration of metoclopramide from 1.0 × 10-7 mol/L to 1.0 × 10-4 mol/L and the detection limit （S/N = 3） was 1.40 × 10-9mol/L. Through 7-timeparallel determination of 1.0× 10 -5mol/L metoclopramide, the RSD of results was 1.8%. The recovery ranging from 98.3% to 104. 4% was obtained in the detection of metoclopramide tablets and the RSD was 2. 3% （ n = 5）. The proposed method shows high selectivity and sensitivity and simplicity, and it could be applied to the analysis of metoclopramide with satisfactory results.

关 键 词：电致化学发光 联吡啶钌 甲氧氯普胺 纳米金 硅溶胶 

分 类 号：O657.3[理学—分析化学]