rLukS-PV体外诱导人急性髓系白血病细胞THP-1分化作用研究  

rLukS-PV induces differentiation function in human acute myeloid leukemia THP-1 cells in vitro

作　　者：章成芳 马筱玲[1] 戴春阳[1] 孙晓曦[1] 

机构地区：[1]安徽医科大学附属省立医院检验科,合肥230001

出　　处：《安徽医科大学学报》2015年第3期280-285,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1408085MH188)

摘　　要：目的研究金黄色葡萄球菌PV-杀白细胞毒素亚组分(Luk S-PV)对人急性髓系白血病细胞THP-1的诱导分化作用,为寻求白血病新的靶向治疗药物奠定基础。方法分别采用0、0.50、1.00、1.50μmol/L体外重组PV-杀白细胞毒素亚组分Luk S-PV(r Luk S-PV)体外刺激THP-1细胞24、48h。倒置显微镜、瑞氏-吉萨姆染色镜检等方法观察r Luk SPV作用前后细胞的形态学变化,流式细胞术检测细胞表面特异性抗原CD11b、CD14,荧光显微镜观察细胞吞噬功能。结果经r Luk S-PV刺激的THP-1细胞由分散、悬浮转变为黏附、贴壁,且呈梭形。细胞体积增大,胞质增多,核质比例缩小,细胞核呈肾形、三角形或不规则形,偏于一侧。细胞表面CD11b、CD14表达增加,以CD14增加较为显著,细胞对荧光颗粒吞噬功能明显增强。以上作用有明显的时间和剂量依赖性。结论 r Luk S-PV具有诱导THP-1细胞向单核-巨噬细胞定向分化的作用,有望成为一种新的髓系白血病靶向治疗药物。Objective To investigate the induction differentiation effect of the subunit of Panton-Valentine leukoci- din （LukS-PV） on the acute myeloid leukemia THP-1 cell lines and search a promising therapeutic strategy of mye- loid leukemia. Methods THP-1 cells were treated with different concentrations （0,0.50,1.00,1.50 μmoL/L） of recombinant LukS-PV （rLukS-PV）. After 24, 48 h, the morphology of induced cells was observed with Wright-Gi- emsa staining under optical microscope. The expression of differentiation markers CD14 and CD1 lb was determined by flow cytometry. The cell phagocytosis of fluorescent particles was examined by fluorescence microscope. Results THP-1 cells treated with rLukS were changed from dispersal and suspended into adhesive and fusiform. Cell vol- ume and cytoplasm content increased, nucleo-cytoplasmic ratio decreased. The shape of cell nucleus was reniform, triangle and irregular. The cell nucleus was located at the side of cells. The expression of CDllb and CD14 was significantly increased, especially CD14. The cell phagocytosis of fluorescent particles was also obviously en- hanced. The above effects were both in a dose- and time-dependent manner. Conclusion rLukS-PV has the dif- ferentiation effect of inducing THP-1 cell lines into monocyte-macrophages system. These findings suggest the rLukS-PV maybe as a novel approach for treatment of myeloid leukemia.

关 键 词：PV-杀白细胞毒素 THP-1细胞 诱导分化 

分 类 号：R733.713[医药卫生—肿瘤]