流式细胞术检测血小板脱糖方法建立及意义  

The test method about desialylation of platelet to establish and its significance

作　　者：汪嘉佳 陶莉莉[1] 潘莹[1] 王会平[1] 晏开力[1] 张家奎[1] 倪合宇[2] 翟志敏[1] 

机构地区：[1]安徽医科大学第二附属医院血液内科(安徽医科大学血液病研究中心),合肥230601 [2]加拿大多伦多大学实验医学与病理生物学系

出　　处：《安徽医科大学学报》2015年第3期329-332,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81141104);安徽高校省级自然科学研究重大项目(编号:KJ2014Z017)

摘　　要：目的通过运用流式细胞术检测正常人血小板在4、25℃下,0、4、8、24 h各个时间点,血小板脱糖的两个标志物蓖麻凝集素(RCA)、鸡冠刺桐凝集素(ECL)是否有所差异,确立运用流式细胞术检测血小板脱糖水平的最佳条件。确立最佳条件后,运用流式细胞术检测20例原发免疫性血小板减少症(ITP)患者的血小板脱糖水平。方法随机选取正常成年人20例,运用多参数流式细胞术检测正常成年人不同温度(4、25℃),不同时间点(0、4、8、24 h)条件下血小板脱糖水平(RCA、ECL)是否存在差异。确立最佳条件后,运用流式细胞术检测20例ITP患者血小板脱糖水平。结果4、25℃下,4个时间点脱糖水平RCA%、ECL%均不明显。常规治疗效果较好的ITP患者脱糖水平RCA%、ECL%不明显,但是治疗效果不好的ITP患者脱糖水平RCA%、ECL%明显高于治疗效果理想的患者。结论流式细胞术检测血小板脱糖水平最好在标本收集后立即处理,脱糖水平(RCA、ECL)与ITP患者治疗效果有关,即脱糖水平越低其治疗效果越好。Objective To detect desialylation of platelets in normal,and research influence about ricinus communis agglutinin（RCA） and erythrina cristagalli lectin（ECL） which are two factors of desialylation of platelets in different time periods（0,4,8,24 h） and different temperatures（4,25 ℃）,thus establish the test method which can detect desialylation of platelets in autoimmune thrombocytopenia（ ITP） . Methods 20 normal subjects were selected and researched for the differences about RCA and ECL which were two factors of desialyation of platelets in different time periods（0,4,8,24 h） and different temperatures（4,25℃） with flow cytometry. Under this condition, the best test method that could detect desialylation of platelets in 20 cases of ITP could be established in the end. Results The results showed that the median of the desialylation of platelets（ RCA% and ECL%） was not significant in 4 pe-riods under 4,25 ℃,respectively. The results showed that the median of the desialylation of platelets（ RCA% and ECL%） in effective responses to conventional therapy（ corticosteroids,IVIG etc） was lower than the group which were inferior responses to conventional therapy. Conclusion The test method about desialylation of platelets in ITP should be better to detect in one day. The desialylation of platelets（RCA,ECL） is interrelated with treatment of ITP. And the lower desialyation prompts better outcome in ITP which is effective response to conventional therapy.

关 键 词：血小板脱糖 原发免疫性血小板减少症 RCA ECL 流式细胞术 

分 类 号：R733.711[医药卫生—肿瘤]