黄绿蜜环菌菌丝体多糖分离纯化与含量测定  

Isolation,purification and content determination of polysaccharide in Armillaria luteo-rivens mycelium

作　　者：史强强[1,2] 党军[1] 岳会兰[1] 文怀秀[1] 陶燕铎[1] 王启兰[1] 

机构地区：[1]中国科学院西北高原生物研究所中国科学院藏药研究重点实验室,西宁810008 [2]中国科学院大学,北京100049

出　　处：《工业微生物》2015年第2期35-38,共4页Industrial Microbiology

基　　金：青海省高新技术研究与发展计划(2012-G-218)

摘　　要：分离纯化黄绿蜜环菌菌丝体粗多糖,提高多糖含量,以进一步应用于工业化生产。采用DEAE-52纤维素层析柱分离纯化黄绿蜜环菌菌丝体多糖,依次用水、0.1 mol/L Na Cl、0.2 mol/L Na Cl、0.4 mol/L Na Cl、0.8 mol/L Na Cl进行洗脱;苯酚-硫酸法测定纯化后多糖含量,测定波长490nm,葡萄糖浓度与吸光度的回归方程为A=6.3137X+0.2156R2=0.9909。黄绿蜜环菌菌丝体水体粗多糖经DEAE-52纤维素层析柱分离纯化后,多糖含量达到99%以上。经过中试试验验证提取及分离纯化工艺适用于工业化生产。In order to enhance the content of polysaccharide,isolation and purification of polysaccharide of Armillaria luteo-rivens mycelium were conducted for the application to industrial production. The polysaccharide in Armillaria luteo-rivens mycelium was separated and purified by DEAE-52,and eluted gradiently by water,0. 1 mol/L NaCl,0. 2 mol/L NaCl,0. 4 mol/L NaCl and 0. 8 mol/L NaCl;the content of polysaccharide was measured by the phenol-sulfuric acid method,determined with wavelength of 490 nm. The linear relationship of glucose concentration was good with regression equation A=6. 3137X＋0. 2156（R2 =0. 9909）. The content of polysaccharide exceeded 99% after isolation and purifica-tion. The results showed that the process of extraction,separation and purification was suitable for industrial production.

关 键 词：黄绿蜜环菌菌丝体 多糖 DEAE-52 苯酚-硫酸法 

分 类 号：TQ920[轻工技术与工程—发酵工程]