Shc1在肝癌组织中的表达及对肝癌SMMC-7721细胞增殖迁移能力的影响  

Expression of Shc1 in hepatocellular carcinoma tissues and its effect on proliferation and migration of human hepatocarcinoma SMMC-7721 cells

作　　者：戴敏[1] 刘俊[1] 朱海燕[2] 顾澄宇[3] 陶汉川 乔谦[3] 杨树东[4] 蔡兵[3] 

机构地区：[1]南通大学附属海安医院普通外科,南通226600 [2]南通大学附属海安医院肿瘤科,南通226600 [3]南京医科大学附属无锡市人民医院肝胆外科,无锡214000 [4]南京医科大学附属无锡市人民医院病理科,无锡214000

出　　处：《第二军医大学学报》2014年第9期944-951,共8页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金青年项目(81302105);无锡市科技计划项目(CYE00917)~~

摘　　要：目的探讨Shc1在肝癌组织中的表达情况及其对人肝癌SMMC-7721细胞增殖、迁移能力的影响。方法采用实时定量PCR、蛋白质印迹法、免疫组织化学法检测33例肝癌及其对应的癌旁组织中Shc1的表达情况,并分析Shc1蛋白表达量与临床特征间的关系;培养肝癌SMMC-7721细胞,以siRNA技术干扰Shc1的表达,采用MTT法和细胞划痕试验分别检测干扰Shc1对细胞增殖活力和迁移能力的影响。结果在33对样本中,肝癌组织中Shc1的表达高于癌旁组织(P<0.05);Shc1表达于肝癌细胞的胞质中。无肝硬化的患者Shc1阳性率(100%)高于伴肝硬化者(54.2%),术前Child-Pugh分级A级的患者Shc1阳性率(75.9%)高于B级或C级的患者(0%),术前血AFP阳性患者Shc1阳性率(79.2%)高于AFP阴性患者(33.3%),术后病理EdmondsonⅢ、Ⅳ级患者Shc1阳性率(84.2%)高于Ⅰ、Ⅱ级的患者(42.9%),差异均有统计学意义(P<0.05)。干扰Shc1表达后,随时间延长,SMMC-7721细胞增殖、迁移明显受到抑制(P<0.05)。结论 Shc1在肝癌组织中高表达,且Shc1的阳性率与有无肝硬化、Child-Pugh分级、术前AFP、病理Edmondson分级相关;干扰Shc1的表达可抑制肝癌SMMC-7721细胞的增殖、迁移能力。Objective To investigate the expression of Shc1 in hepatocellular carcinoma tissues and its effect on the proliferation and migration of human hepatocarcinoma SMMC-7721 cells.Methods The expression of Shc1 in 33hepatocellular carcinoma tissues and corresponding adjacent tissues was examined by real-time PCR,Western blotting analysis and immunohistochemistry method;the relationship between the expression and clinical features was also analyzed.Shc1 was silenced by RNA interference technique in SMMC-7721 cells to determine its effect on proliferation and migration;MTT assay and wound healing assay were used to examine the effect of Shc1 knockdown on cell vitality and migration.Results Real-time PCR,Western blotting analysis and immunohistochemistry demonstrated that Shc1 expression in hepatocellular carcinoma tissues was significantly higher than that in the adjacent tissues(P<0.05);the expression of Shc1 was mainly in the cytoplasma.Shc1 positive rates in the patients without cirrhosis(100%),of ChildPugh class A before operation(75.9%),AFP-positive before operation(79.2%)and pathology EdmondsonⅢ/Ⅳlevel after operation(84.2%)were significantly higher than those with cirrhosis(54.2%),of Child-Pugh class B or C(0%),AFP-negative(33.3%),and pathology EdmondsonⅠ/Ⅱ(42.9%,Pall<0.05).Shc1 expression silence resulted in significant decrease in the proliferation and migration of SMMC-7721cells(P<0.05).Conclusion Shc1 is overexpressed in hepatocarcinoma tissue.The expression of Shc1 is associated with cirrhosis,Child-Pugh class,preoperation AFP and pathology Edmondson grades of the hepatocarcinoma.Inhibition of Shc1 expression can inhibit the proliferation and migration of human hepatocarcinoma cell SMMC-7721 in vitro.

关 键 词：Shc1 肝细胞癌 细胞增殖 细胞迁移 RNA干扰 

分 类 号：R735.7[医药卫生—肿瘤]