中国南海黑乳海参共附生真菌Phialemonium sp.次生代谢产物的鉴定  

Identification of secondary metabolites of the fungus Phialemoniumsp.associated with the South China Sea cucumber Holothuria nobilis Selenka

作　　者：王旭东[1] 孙鹏[2] 许东啸 汤华[2] 刘宝姝[2] 张文[2] 

机构地区：[1]解放军第425医院药械科,三亚572000 [2]第二军医大学药学院海洋药物研究中心,上海200433

出　　处：《第二军医大学学报》2014年第9期988-991,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30873200;41176125;81202453);上海浦江人才计划(PJ2008);海南省自然科学基金(811223);三亚市医疗卫生科技创新项目(YW1228)~~

摘　　要：目的对中国南海黑乳海参的共附生单胞瓶霉属真菌(Phialemoniumsp.)的次生代谢产物进行研究。方法运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等分离手段对真菌Phialemoniumsp.的乙酸乙酯提取物进行分离纯化,利用现代波谱技术并结合文献报道进行结构鉴定。结果分离得到2个缩酚酸环醚化合物emeguisin A和aspergillusidone C。结论作为黑乳海参共附生真菌Phialemoniumsp.次生代谢产物,2个化合物均为首次从该真菌中分离得到。Objective To investigate the secondary metabolites of the fungus Phialemoniumsp.associated with the South China Sea cucumber Holothuria nobilis Selenka.Methods The EtOAc extract of Phialemoniumsp.was purified by repeated column chromatography on silica gel,Sephadex LH-20,and high-performance liquid chromatography(HPLC).Structural determination of the isolated compounds was achieved by spectroscopic analysis and by comparison with previously reported data.Results Two depsidones were isolated and their structures were determined as:emeguisin A and aspergillusidone C.Conclusion This is the first report on the secondary metabolites of Phialemoniumsp.associated with the sea cucumber H.nobilis Selenka,and the two depsidones are reported for the first time from the fungus strain Phialemoniumsp.

关 键 词：黑乳海参 海参共附生真菌 单胞瓶霉属 缩酚酸环醚 

分 类 号：R731.77[医药卫生—肿瘤]