茶树根细胞壁不同组分对铅的吸附性能及其功能团的傅里叶红外光谱学研究  

Adsorption ability of cell wall( CW) components in roots of Tea Plant( Camellia sinensis L. ) to Pb and FTIR spectra of their functional groups

作　　者：王梦[1] 段德超[1] 徐辰[1] 于明革[1,2] 施积炎[1] 

机构地区：[1]浙江大学环境与资源学院,杭州310058 [2]杭州市环境集团有限公司,杭州310022

出　　处：《生态学报》2015年第6期1743-1751,共9页Acta Ecologica Sinica

基　　金：国家自然科学基金(41201319);新世纪优秀人才支持计划(NCET-11-0455)

摘　　要：选择我国重要的经济作物之一茶树(Camellia sinensis L.)为研究对象,考察了茶树根细胞壁中不同多糖组分在吸附铅(Pb)过程中的作用差异以及与其发生交互作用的主要功能团。结果表明,在茶树根细胞壁吸附Pb过程中,绝大多数的Pb(68.42%)是吸附在纤维素以及木质素上;其次是果胶(20%)、半纤维素2类(5.26%);半纤维素1类的贡献可以忽略不计。同时,通过细胞壁不同组分吸附Pb前后的傅立叶红外光谱表征结果得出,在吸附Pb的过程中,果胶中起作用的功能团主要有羟基、羧基;半纤维素1类中起作用的功能团主要是羧基;半纤维素2类中起作用的功能团主要为羟基。Tea is one of the most important economic crops in China and the relevant research suggests that every year,Pb content in tea products has shown an increasing trend. Pb is also one of the most important indicators of quality and safety monitoring. Recent,research mostly focused on the resistance of plants to heavy metals was limited to short growing herbs.Reports on the study of woody plants are very rare. On the basis of these conditions tea tree was selected as research work.The objective of the present study is to find out the molecular mechanism of Pb stress resistance in roots CW of tea plants.Tea trees grown in the clean tea garden were selected for our experimental design. Tea roots were collected and washed with deionized water. Then extraction of crude CW and subsequent fractionation of CW components were carried out. Adsorption kinetics was carried out to determine the adsorption ability of different CW components to Pb stress. A total of 5 mg of CW materials or its corresponding residues was placed into a 2-ml column equipped with a filter at the bottom. The solution consisted of 15 μmol / L Pb（ NO3）2in 0. 01 mol / L Na NO3 at p H 5. 0. The solution was sipped by a peristaltic pump set a speed of 8 ml per 10 min after running through a 2-ml column holding the CW samples. The adsorption solutions were collected at 10-min intervals and Pb in the adsorption solutions was measured by Atomic Absorption Spectroscopy（ AAS）.At last the Fourier Transform infrared spectroscopy（ FTIR） spectra of different CW components before and after Pb2 ＋adsorption was carried out to study the difference in functional groups those can interact with Pb between different CW components. The results of this study shown that the vast majority of Pb（68. 42%） adsorbed in the cellulose and lignin,followed by pectin（20%） and hemicellulose2（ HC2）（5. 26%）. While,the contribution of HC1 was negligible. These results indicated that in the CW of roots cellulose and lignin has a greater ability to accumulate Pb

关 键 词：Pb毒 细胞壁组分 傅立叶红外 功能团 茶树 

分 类 号：S571.1[农业科学—茶叶生产加工]