人脐带间充质干细胞裂解液对食管癌EC9706细胞中尿激酶型纤溶酶原激活物和基质金属蛋白酶-2表达的影响  

Effect of lysate from human umbilical cord mesenchymal stem cells on the expression of urokinase plasminogen activator and matrix metalloproteinases-2 in esophageal carcinoma EC9706 cells

作　　者：闫国旗[1] 李汉臣[1] 齐博[1] 朱德奇[1] 赵宝生[1] 

机构地区：[1]新乡医学院第一附属医院胸外科,河南卫辉453100

出　　处：《新乡医学院学报》2015年第4期299-302,共4页Journal of Xinxiang Medical University

基　　金：河南省基础与前沿技术研究计划项目(编号:112300410163)

摘　　要：目的探讨人脐带间充质干细胞(h UCMSCs)裂解液对食管癌EC9706细胞中尿激酶型纤溶酶原激活物(u PA)及基质金属蛋白酶-2(MMP-2)表达的影响。方法收集h UCMSCs,采用反复冻融法制备h UCMSCs裂解液;不同浓度h UCMSCs裂解液作用于食管癌EC9706细胞60 h,对照组加入体积分数10%胎牛血清的培养液。采用Western blot法检测食管癌EC9706细胞中u PA、MMP-2的表达。结果加入h UCMSCs数<食管癌细胞数的裂解液时,食管癌EC9706细胞中u PA、MMP-2的表达与对照组比较差异无统计学意义(P>0.05);加入h UCMSCs数≥食管癌细胞数的裂解液时,食管癌EC9706细胞中u PA、MMP-2的表达较对照组显著降低(P<0.05)。结论 h UCMSCs裂解液对食管癌EC9706细胞的迁移抑制作用可能与u PA、MMP-2有关。Objective To investigate the effect of the lysate from human umbilical cord mesenchymal stem cells（ h UCMSCs） on the expression of urokinase plasminogen activator（ u PA） and matrix metalloproteinases-2（ MMP-2） in esophageal carcinoma EC9706 cells. Methods h UCMSCs were collected and cell lysate was prepared through repeated freeze-thaw cycles. EC9706 cells were treated by cell lysates with different cell numbers of h UCMSCs for 60 hours while the control group was treated with the 10% fetal bovine serum. The expression of u PA and MMP-2 in EC9706 was detected by Western blot. Results The lysate from h UCMSCs with numbers less than treated EC9706 cells did not change both u PA level and MMP-2 level in EC9706 cells when compared with control group（ P 0. 05）. The expression of u PA and MMP-2 in EC9706 cells was decreased significantly compared with control group when the lysate from h UCMSCs with the number of cell was equal or greater than treated EC9706 cells（ P 0. 05）. Conclusion h UCMSCs lysate can inhibit the migration of esophageal carcinoma EC9706 cells,which may be associated with u PA and MMP-2.

关 键 词：人脐带间充质干细胞 食管癌 尿激酶型纤溶酶原激活物 基质金属蛋白酶-2 

分 类 号：R735.1[医药卫生—肿瘤]