肿瘤坏死因子对不同分化程度胃癌细胞增殖及Δ133p53表达的影响  

The Effect of Tumor Necrosis Factor(rmh TNF) on Proliferation and Δ133p53 Expressions in Gastric Cancer Cells with Various Grades

作　　者：张娜[1] 季万胜[2] 杨炳乾[1] 张红梅[2] 仲华[2] 高志星[2] 

机构地区：[1]潍坊医学院,山东潍坊261042 [2]潍坊医学院附属医院,山东潍坊261031

出　　处：《中国肿瘤》2015年第4期335-339,共5页China Cancer

摘　　要：[目的]探讨肿瘤坏死因子(rmh TNF)对低分化人胃癌细胞株MKN45和中分化人胃癌细胞株SGC7901细胞增殖及对细胞中p53异构体Δ133p53表达的影响。[方法]不同浓度注射用重组结构人rmh TNF(0、50IU/ml、100IU/ml、500 IU/ml)作用于人胃癌细胞株MKN45和SGC7901。采用CCK8法检测细胞增殖抑制率;巢式逆转录多聚酶链反应(n RT-PCR)检测Δ133p53及下游分子PTEN在m RNA水平的表达情况。Pearson直线相关分析Δ133p53与PTEN m RNA表达的相关性。[结果 ]CCK8法结果显示,rmh TNF对MKN45胃癌细胞株的生长有显著的增殖抑制作用(H=15.434,P=0.001),实验组细胞(50IU/ml、100IU/ml、500 IU/ml)的增殖抑制率分别为26.2%,33.62%,48.49%。rmh TNF对SGC7901胃癌细胞株的生长无明显增殖抑制作用,实验组细胞的增殖抑制率分别为4.02%,4.63%和2.68%。n RT-PCR结果显示,随rmh TNF浓度的增加,MKN45胃癌细胞株中Δ133p53在m RNA水平的相对表达量减少,PTEN的相对表达量增加,差异均有统计学意义(H=10.385,P=0.016)。SGC7901胃癌细胞株中无Δ133p53表达,PTEN的表达不随rmh TNF浓度的变化而变化。MKN45胃癌细胞株中Δ133p53与PTEN的表达呈负相关(r=-0.958,P<0.01)。[结论]rmh TNF对胃癌细胞的抑制作用与Δ133p53异构体参与的TNF-NF-κB和p53-PTEN信号传导途径的交互作用有关,提示Δ133p53异构体可能是胃癌分子诊断和生物学治疗的重要靶分子之一。[Purpose] To investigate the effect of tumor necrosis factor on proliferation andΔ133p53 expression in human poorly differentiated gastric carcinoma cell line MKN45 and middle differentiated gastric carcinoma cell line SGC7901. [Methods]The different concentrations of rmh TNF（0,50 IU/ml,100 IU/ml,500 IU/ml） effected on the gastric carcinoma cell lines MKN45 and SGC7901. The growth inhibitory effect of rmh TNF on human gastric carcinoma cell lines was observed by CCK8 assay. The expression of p53 isoform Δ133p53 and p53 downstream molecule PTEN on the m RNA level was detected by nested reverse transcription polymerase chain reaction（n RT-PCR）. The relationship between Δ133p53 and PTEN m RNA was analyzed by Pearson linear analysis. [Results] In CCK8 assay,it was shown that the MKN45 gastric carcinoma cell line were significantly inhibited by rmh TNF,with significant difference（H=15.434,P=0.001）;the growth inhibitory effect of experiment cell groups（50IU/ml、100IU/ml、500 IU/ml）was 26.2%,33.62% and48.49% respectively. The SGC7901 gastric carcinoma cell line was not inhibited by rmh TNF,and the growth inhibitory effect of experiment cell group was 4.02%,4.63% and 2.68% respectively.n RT-PCR results showed that with the rmh TNF concentration increased,the expression ofΔ133p53 were gradually decreased and the expression of PTEN were gradually increased on m RNA levels in the MKN45 gastric carcinoma cell line（H=10.385,P=0.016）. There was no Δ133p53expression in SGC7901 gastric carcinoma cell line and the PTEN ＇s relative expression was no significant change. The Δ133p53 m RNA expression was negatively related to PTEN m RNA expression in MKN45 gastric carcinoma cell line（r=-0.958,P〈0.01）. [Conclusion] The inhibitory effect of rmh TNF to gastric carcinoma was associated with the cross-talk between TNF-NF-κB and p53PTEN pathway,in which Δ133p53 isoform plays a pivotal role. The result indicated that theΔ133p53 isoform might be an important target molecular for the molecular diagnosis

关 键 词：肿瘤坏死因子 胃癌 p53异构体 Δ133p53 PTEN 

分 类 号：R735.2[医药卫生—肿瘤]