纤维素酶提取金钗石斛多糖工艺研究  

Cellulase Extraction Technology of Polysaccharide from Dendrobium Nobile

作　　者：张晓敏[1] 孙志蓉[1] 朱南南[1] 程丽丽[1] 杨春宁[1] 王晨[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中医药信息》2015年第2期50-53,共4页Information on Traditional Chinese Medicine

基　　金："十一五"国家科技支撑计划项目(No.2009BAI74B02-3)

摘　　要：目的:对纤维素酶用于金钗石斛多糖提取工艺的酶解参数进行优选。方法:通过正交实验,以多糖提取率为指标,采用紫外分光光度法测定多糖含量,以此评价酶解工艺的优劣。结果:最佳酶解条件为:料液比1∶50,pH值5.0,提取时间170min,酶用量4500U/g,酶解温度55℃,此时金钗石斛多糖提取率为7.71%,比常规热回流提取方法高43.84%。结论:本方法装置简单,提取率高,为金钗石斛深入研究奠定基础,在中药有效成分的提取方面具有潜在的工业应用价值。Objective： To optimize the parameters of the cellulose extraction of Polysaccharide from Dendrobium nobile.Mothods： Regard the extraction rate of polysaccharide as indexes,and use orthogonal experriments to determine the content of index in cellulose by UV Spectrophotometry.Then evaluate the merits of enzymatic parameters.Results： The optimized process conditions were solid-to-liquid ratio 1： 50,pH 5.0,reflux time170 min,dosage of enzyme 4500U/g,enzymolysis temperature 55℃,and the Dendrobium nobile polysaccharide extraction rate was 7.71%,with 43.84% higher than conventional thermal reflow method.Conclusion： This method has advantages of simplicity,high extraction rate,which lays a foundation for the further study of Dendrobium nobile.It also has the potentially industrial application value in the extraction of effective ingredients of Chinese Medicine.

关 键 词：金钗石斛 多糖 正交实验 纤维素酶 

分 类 号：R284.2[医药卫生—中药学]