枣60S核糖体蛋白L8-3基因的克隆及生物信息学分析  

Cloning and bioinformatic analysis of 60S ribosomal protein L8-3 gene fromZiziphus jujuba

作　　者：卜娇迪 陈莹莹[2] 刘孟军[2] 赵锦[1] 

机构地区：[1]河北农业大学生命学院,河北保定071000 [2]河北农业大学中国枣研究中心,河北保定071000

出　　处：《河北农业大学学报》2015年第2期72-75,98,共5页Journal of Hebei Agricultural University

基　　金：国家科技支撑项目(2013BAD14B03);河北省科技支撑计划(11230606D-6)

摘　　要：核糖体是细胞进行蛋白质合成的重要场所。为了解枣60S核糖体蛋白L8-3基因的结构,以‘月光’枣为材料,根据GenBank中登录的苹果、桃和柑橘等植物60S核糖体蛋白保守序列设计引物,采用RT-PCR法,从枣叶片中克隆出该基因,并对其编码蛋白进行了生物信息学分析。生物信息学分析表明:该基因包含完整的开放阅读框为783bp,编码260个氨基酸残基,预测分子量为28.025kDa,理论等电点为10.83,命名为ZjRPL8-3(登录号:KM106202);保守结构域分析表明,该序列属于L2超家族蛋白;分子进化树显示,枣ZjRPL8-3与蔷薇科植物中该基因的蛋白序列亲缘关系最近,此结果一定程度上反应了物种间的亲缘关系。The ribosome is responsible for the synthesis of proteins in cells. To understand the structure of 60S ribosomal protein L8-3 gene in Chinese jujube, Ziziphusjujuba Mill. ＇Yue- guang＇ was used as the material to clone the gene. A 783 bp open reading frame cDNA enco- ding 60S ribosomal protein was cloned by RT-PCR method on the basis of the homologous genes of Malus domestica, Prunus persica and Citrus clementina, etc. in GenBank. The se- quence was named ZjRPLS-3 （accession number KM106202） and encoded 260 amino acids, with molecular weight 28. 025 kDa and theoretical isoelectric point 10.83. Conservation analy- sis of ZjRPLS-3 showed that it belonged to the L2 family. Phylogenic analysis showed that 60S ribosomal protein from jujube had a closer relationship with that of the family Rosaceae.

关 键 词：枣 核糖体蛋白 克隆 生物信息学分析 

分 类 号：Q785[生物学—分子生物学]