AMPA受体分布对帕金森病及异动症发生的影响  

Effects of distribution of AMPA receptors on incidence of Parkinson disease and dyskinesia

作　　者：干静[1] 戚辰[1] 章素芳[1] 刘振国[1] 

机构地区：[1]上海交通大学医学院附属新华医院神经内科,上海200092

出　　处：《上海交通大学学报（医学版）》2015年第3期308-315,共8页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81171203;81200871;81400925);上海市科技委员会项目(12XD1403800)~~

摘　　要：目的观察α-氨基-3-羟基-5-甲基-4-异唑丙酸(AMPA)受体各亚型在帕金森病(PD)及异动症[左旋多巴诱发异动症(LID)]大鼠模型纹状体中亚细胞分布的变化。方法应用6-羟基多巴(6-OHDA)制备PD大鼠模型,向PD大鼠腹腔注射左旋多巴建立LID大鼠模型。引入受体蛋白质交联方法,通过Western blotting检测大鼠纹状体神经元AMPA受体各亚型在细胞表面和细胞内池不同部位含量变化。结果在PD大鼠纹状体中,AMPA受体各亚型均未发生亚细胞分布改变;慢性左旋多巴刺激诱导大鼠LID对其纹状体AMPA总蛋白以及在细胞表面和细胞内池的分布无明显影响。结论 AMPA受体分布变化对PD或LID的发生无明显影响。Objective To observe changes of the subcellular distribution of subtypes of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate（ AMPA） receptors in the striatum of Parkinson disease（ PD） and dyskinesia[levodopa-induced dyskinesia（ LID） ] rat model. Methods Neurotoxin 6-hydroxydoapmine（ 6-OHDA） was used to establish the PD rat model. PD rats were intraperitoneally injected with levodopa to establish the LID rat model.The surface receptor cross-linking assay was adopted and changes of levels of AMPA receptor subtypes at different locations of surface pools and intracellular pools of striatal neurons were detected by the Western blotting. Results The subcellular distribution of subtypes of AMPA receptors in the striatum of PD rats showed no changes. AMPA total protein and the distribution of AMPA in surface pools and intracellular pools of the striatum of LID rats were not significantly affected by the chronic induction of levodopa. Conclusion Changes of the distribution of AMPA receptors have no significant effect on the incidence of PD or LID.

关 键 词：帕金森病 异动症 α-氨基-3-羟基-5-甲基-4-异唑丙酸受体 纹状体 

分 类 号：R742.5[医药卫生—神经病学与精神病学]