循环miR-4677在口腔鳞状细胞癌患者中的表达  

Expression of circulating miR-4677 of patients with oral squamous cell carcinoma

作　　者：顾文莉[1] 吴静静[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院检验科,上海200011

出　　处：《上海交通大学学报（医学版）》2015年第3期348-352,357,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81101642)~~

摘　　要：目的观察循环微RNA-4677(miR-4677)在口腔鳞状细胞癌(OSCC)患者中的表达,探讨其临床应用价值。方法通过茎环反转录法及real-time PCR技术,检测83例OSCC患者(OSCC组)和44名健康对照者(正常对照组)血浆中miR-4677的表达水平,评估其作为OSCC诊断标志的检测效能。结果茎环反转录PCR法可特异扩增循环miR-4677;与正常对照组比较,OSCC组血浆miR-4677表达水平显著降低(P<0.01);受试者工作特征曲线(ROC曲线)分析显示血浆miR-4677水平可有效区分OSCC患者与正常对照人群,其曲线下面积(AUCROC)可达0.882;当确定最佳截断值为0.625(fold值)时,血浆中的循环miR-4677对OSCC的诊断敏感度和特异度分别达到79.5%和91.6%。结论循环miR-4677对于OSCC患者具有较好的诊断效能,有望成为OSCC早期诊断的分子标志物,为肿瘤的基因诊断开拓了一条新思路。Objective To observe the expression of circulating microRNA-4677（ miR-4677） of patients with oral squamous cell carcinoma（ OSCC） and explore the clinical significance. Methods The expression of plasma miR-4677 of 83 patients with OSCC（ OSCC group） and 44 healthy controls（ control group） were detected by the stem-loop RT and real-time PCR. The diagnostic performance of using the miR-4677 expression level as OSCC biomarker was evaluated. Results Stem-loop RT-PCR could specifically amplify miR-4677. Compared with the control group,the expression of plasma miR-4677 of the OSCC group was significantly lower（ P〈0. 01）. The results of receiver operating characteristic（ ROC） curve showed that the plasma miR-4677 level could efficiently distinguish patients with OSCC from healthy people and AUCROCwas 0. 882. When the optimal cutoff value of 0. 625（ fold value） was chosen, the sensitivity and specificity of plasma miR-4677 for diagnosing the OSCC were 79. 5% and 91. 6%,respectively. Conclusion The circulating miR-4677 is satisfactory for diagnosing the OSCC and can be used as the biomarker for early diagnosis of OSCC. It is a novel idea of genetic diagnosis of cancer.

关 键 词：口腔鳞状细胞癌 微RNA-4677 循环 血浆检测 肿瘤标志物 

分 类 号：R739.8[医药卫生—肿瘤]