肝癌组织DLK1和Jagged1表达临床意义探讨  

Correlation analysis and clinic significance of DLK1 and Jagged1 in hepatocellular carcinoma tissues

作　　者：欧阳合意[1] 张艳丽[1] 陈小伍[1] 王卫东[1] 康凯夫[1] 

机构地区：[1]南方医科大学附属顺德第一人民医院病理科,广东顺德528300

出　　处：《中华肿瘤防治杂志》2015年第8期610-613,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：广东省社会发展领域科技计划(2010B031500003)

摘　　要：目的探讨DLK1和Jagged1在原发性肝细胞性肝癌组织中的表达情况,深入分析两者与肝癌临床病理资料的关系及其临床意义。方法收集2001-01-01-2009-10-30顺德第一人民医院病理科采用免疫组化SP法检查10例正常肝组织、50例肝炎、50例肝硬化和150例肝细胞性肝癌组织中DLK1和Jagged1的表达情况。结果 DLK1阳性表达率在正常肝组织为0(0/10)、肝炎组为6.00%(3/50)、肝硬化组为28.00%(14/50)、肝癌组为46.67%(70/150),四组间阳性率比较差异有统计学意义,χ2=34.38,P<0.05。正常肝组织和肝炎组,正常肝组织和肝硬化组,正常肝组织和肝癌组,肝硬化和肝癌组两两比较差异均无统计学意义,P值均>0.05。肝炎和肝硬化组(χ2=8.575,P=0.003),肝炎和肝癌组(χ2=26.757,P<0.001)两两比较差异均有统计学意义。Jagged1阳性表达率在正常肝组织中为40.00%(4/10)、肝炎组为40.00%(20/50)、肝硬化组为62.00%(31/50)、肝癌组为71.33%(107/150),四组间阳性率比较差异有统计学意义,χ2=17.92,P<0.05。正常肝组织和肝炎组,正常肝组织和肝硬化组,正常肝组织和肝癌组,肝炎组和肝硬化组,肝硬化组和肝癌组,两两比较差异均无统计学意义,P值均>0.05。肝炎组和肝癌组比较差异有统计学意义,χ2=15.885,P<0.001。DLK1表达与年龄有关(χ2=15.01,P<0.05),Jagged1表达与年龄(χ2=7.94,P<0.05)、分化程度(χ2=14.79,P<0.05)、HBsAg(χ2=4.53,P<0.05)和预后(χ2=3.92,P<0.05)有关。DLK1和Jagged1在肝细胞癌组织中表达密切相关,χ2=12.79,P<0.05。结论 DLK1和Jagged1的异常表达与肝癌的发生、发展和分化密切相关。OBJECTIVE To explore the expression of DLK1 and Jagged1 in human hepatocellular carcinoma,and the relationship between the expression and clinicopathological characters. METHODS The sample sources were from De- partment of Pathology,First People＇s Hospital of Shunde from January 1 2001 to October 30 2009. The expressions of DLK1 and .laggedl in 10 normal liver tissues, 50 hepatitis tissues,50 cirrhosis tissues and 150 hepatocellular carcinoma tis- sues were detected by immunohistochemical method. RESULTS The expression of DLK1 in normal liver tissues was 0（0/10）,hepatitis tissues 6. 00% （3/50）, cirrhosis tissues 28. 00% （14/50）, hepatocellular cancer tissues 46. 67% （70/150） ,the differences were statistically significant（xz = 34.38, P^0.05）. In normal liver tissue and hepatitis tissue groups,normal liver tissue and cirrhosis tissue groups; normal liver tissue and hepatocellular cancer tissue groups, cirrho- sis tissues and hepatoeellular cancer tissues groups, there were no signifeant difference, all P values 〉0.05. In hepatitis tissues and cirrhosis tissues groups（X2= 8. 575, P = 0. 003）, hepatitis tissues and hepatoeellular cancer tissues groups （X2 = 26. 757, P〈0. 001） ,the differences were statistically significant. The expression of Jagged1 in normal liver tissues were 40.00% （4/10）, hepatitis tissues 40.00 % （ 20/50）, cirrhosis tissues 62.00 % （ 31/50 ）, hepatocellular cancer tissues 71.33%（107/150） ,the differences among the four were statistically significant （X2 =17.92 ,P〈0. 05）. In normal liver tis- sue and hepatitis tissue groups, normal liver tissue and cirrhosis tissue groups, normal liver tissue and hepatocellular cancer tissue groups,hepatitis tissue and cirrhosis tissue groups, cirrhosis tissue and hepatocellular cancer tissue groups, there were no signifcant difference,all P values 〉0.05. In hepatitis tissue and hepatocellular cancer tissue groups（X2 =15. 885,P〈0. 001）,the differences were statistically significant. The DLK1 express

关 键 词：DLK1 JAGGED1 肝细胞性肝癌 分化 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]