Vav3基因对人胃癌 SGC7901细胞增殖能力的影响及其机制  

Effect and mechanism of Vav3 on the proliferation of human gastric cancer SGC7901 cells

作　　者：檀碧波[1] 李勇[1] 范立侨[1] 赵群[1] 王冬[1] 刘羽[1] 李兆星[1] 

机构地区：[1]河北医科大学第四医院外三科,石家庄050011

出　　处：《中华肿瘤杂志》2015年第3期175-180,共6页Chinese Journal of Oncology

基　　金：国家自然科学基金（81072033、81372580）;河北省自然科学基金（C2010000619）;河北省普通高校强势特色学科资助项目（冀教高[2005]52）;河北省科技支撑项目（14277779D）;河北省卫生厅重大医学科研课题（zd2013040）

摘　　要：目的：探讨Vav3基因对人胃癌SGC7901细胞增殖能力的影响及其机制。方法采用Western blot法检测胃癌SGC7901细胞、胃上皮GES－1细胞、胃癌组织和癌旁组织中Vav3蛋白的表达。将Vav3－siRNA转染胃癌SGC7901细胞，采用四甲基偶氮唑蓝法检测胃癌细胞的增殖，流式细胞术检测细胞周期，实时荧光定量PCR和Western blot法检测增殖相关基因增殖细胞核抗原（ PCNA）、p16、cyclin D1和Rb的表达。制作胃癌原位移植瘤裸鼠模型，检测各组裸鼠的肿瘤生长情况及肿瘤组织中PCNA、p16、cyclin D1和Rb蛋白的表达。结果 Vav3蛋白在胃癌组织和癌旁组织中的相对表达量分别为0．910±0．242和0．243±0．045；在胃癌SGC7901细胞和胃上皮GSE－1细胞中的相对表达量分别为0．925±0．127和0．277±0．038，差异均有统计学意义（均P＜0．05）。 Vav3－siRNA转染可成功抑制SGC7901细胞中Vav3蛋白的表达。以80 nmol／L Vav3－siRNA转染SGC7901细胞72 h后， SGC7901细胞的增殖抑制率达（83．43±10．17）％。 Vav3－siRNA转染后，SGC7901细胞中G0／G1期细胞比例增加，S期细胞明显减少（ P＜0．05）；PCNA和cyclin D1的表达明显下降，而p16和Rb的表达则明显升高（均P＜0．05）。 Vav3－siRNA转染可明显抑制裸鼠肿瘤的生长，并使肿瘤组织中PCNA、cyclin D1蛋白的表达下降，p16和Rb蛋白的表达升高（P＜0．05）。结论 Vav3通过调节增殖相关基因的表达促进胃癌细胞的增殖。Objective The purpose of this study was to investigate the effect and mechanism of Vav3 gene on the proliferation of human gastric cancer cell line SGC7901.Methods The expressions of Vav3 proten in gastric cancer tissue, tumor-adjacent tissue, human gastric cancer cell line SGC7901 and gastric epithelial cell line GES-1 cells were tested by Western blot.Vav3-siRNA was transfected into the SGC7901 cells.The proliferation of SGC7901 cells in vitro was measured by MTT assay.Cell cycle of SGC7901 cells was determined by flow cytometry.The expressions of proliferation-related genes PCNA, p16, cyclin D1, Rb were determined by qPCR and Western blot assay.Orthotopic transplantation nude mouse models of gastric cancer were prepared, and the tumor growth and expressions of PCNA, P16, cyclin D1, and Rb proteins were examined.Results The relative expressions of Vav3 in the gastric cancer and peritumoral tissue were 0.910 ±0.242 and 0.243 ±0.045, respectively;the relative expressions of Vav3 in SGC7901 and GSE-1 cells were 0.925 ±0.127 and 0.277 ±0.038, respevtively （both P〈0.05）.The expression of Vav3 protein in SGC7901 cells was effectively inhibited by Vav3-siRNA.Proliferation of SGC7901 cells was inhibited by （83.43 ±10.17）%after 80 nmol/L Vav3-siRNA transfection （P〈0.05） . The ratio of SGC7901 cells in G0/G1 phase was increased, and in S phase decreased after Vav3-siRNA transfection （both P〈0.05）.The expressions of PCNA and cyclin D1 were decreased in cells after Vav3-siRNA transfection, and expressions of p16 and Rb were increased after Vav3-siRNA transfection （P〈0.05 for all） .The tumor growth in the Vav3-siRNA group was much slower than that in the other 2 control groups＇nbsp;of nude mouse models.Compared with the two control groups, expressions of PCNA and cyclin D1 were significantly lower in the Vav3-siRNA group, while expressions of p16 and Rb were increased （P〈0.05 for all） .Conclusion Vav3 can promote the proliferation of gastric cancer cells by regulating proliferation-r

关 键 词：胃肿瘤 Vav3基因 细胞增殖 RNA干扰 裸鼠 

分 类 号：R735.2[医药卫生—肿瘤]