早期糖尿病大鼠心肌Wnt/β-catenin信号通路的变化  

Alteration of Wnt/β-catenin signaling pathway in early diabetic rat myocardium

作　　者：席晓慧[1,2] 王福文[1] 王燕[1] 尹茂山 牟艳玲[1] 

机构地区：[1]山东省医学科学院药物研究所 [2]济南大学、山东省医学科学院医学与生命科学学院,山东济南250062

出　　处：《中国药理学通报》2015年第3期363-366,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30701022);山东省优秀中青年科学家科研奖励基金计划项目(No BS2013SW008)

摘　　要：目的检测Wnt/β-catenin信号通路相关蛋白在早期糖尿病大鼠心肌中的表达变化,明确其在糖尿病心肌病发生发展中的作用。方法采用链脲佐菌素(60μg·g-1)一次性腹腔注射建立1型糖尿病大鼠模型,喂养2周,HE染色法观察心肌病理结构变化,Western blot和免疫组化法检测心肌Wnt2、β-catenin、c-Myc和DKK1蛋白的表达变化。结果显微镜下糖尿病组大鼠心肌可见局灶性心肌细胞变性、坏死。糖尿病组大鼠相对于正常对照组心肌Wnt2、β-catenin和c-Myc的表达明显增加(P<0.01),而DKK1的表达与正常对照组相比没有明显差异(P=0.885)。结论 Wnt/β-catenin信号通路的激活参与早期糖尿病心肌损伤,进一步研究其在糖尿病心肌中的变化可能为糖尿病心肌病找到新的治疗靶点。Aim To investigate the alteration of Wnt/β-catenin signaling pathway in early diabetic rat myo-cardium and clarify its role in development of diabetic cardiomyopathy. Methods The diabetes mellitus （ DM） model was prepared by intraperitoneal injection of streptozotocin （ STZ, 60 μg · g-1 ） . The alteration of Wnt/β-catenin signaling pathway was determined by Western blot and immunohistochemistry. HE staining was used to analyze the change of myocardial pathologi-cal structure. Results Cardiac histological analyses revealed that DM induced cardiomyocyte degeneration and necrosis. Myocardial Wnt2, β-catenin and c-Myc were enhanced in 2 wk DM compared with control group while DKK1 showed no significant alteration. Conclusion Wnt/β-catenin signaling pathway is acti-vated in early diabetic myocardial injury. Further re-searches on its role in DM myocardium may find a new therapeutic target for diabetic cardiomyopathy.

关 键 词：Wnt2 Β-CATENIN DKK1 C-MYC 糖尿病 心肌损伤 链脲佐菌素 

分 类 号：R-332[医药卫生] R322.11