黄芪多糖对异丙肾上腺素诱发的SERCA2a表达及活性降低的影响  

Effect of astragalus polysaccharide on isopreterenol induced SERCA2a expression and activity downregulation

作　　者：戴红良[1,2] 贾桂枝[3] 赵颂[4] 赵艳[4] 张晓红[3] 王洪新[2] 

机构地区：[1]辽宁医学院护理学院,辽宁锦州121001 [2]辽宁医学院辽宁省心脑血管药物研究重点实验室,辽宁锦州121001 [3]辽宁医学院生物化学与分子生物学教研室,辽宁锦州121001 [4]辽宁医学院科学实验中心,辽宁锦州121001

出　　处：《中国药理学通报》2015年第3期388-391,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30973898)

摘　　要：目的探讨黄芪多糖对异丙肾上腺素(isopreterenol,ISO)刺激下心肌细胞SERCA2a表达的影响。方法采用Real-time PCR法检测SERCA2a mRNA的表达;利用蛋白质免疫印迹法检测SERCA2a蛋白的表达。以4-硝基酚磷酸二钠(p-NPP)法检测SERCA2a的活性。结果与对照组相比,ISO组心肌细胞SERCA2a mRNA及蛋白的表达均明显降低,而黄芪多糖剂量依赖性地逆转上述降低。此外,黄芪多糖还可明显提高SERCA2a的活性。结论黄芪多糖可缓解ISO引起的SERCA2a表达及活性的下调。该作用可能是黄芪多糖缓解心肌细胞内Ca2+超载,进而发挥其心血管保护作用的重要机制。Aim To explore the effect of astragalus polysaccharide （APS） on SERCA2a expression and activity in cardiac myocytes under isopreterenol （ISO） stimulation. Methods SERCA2a mRNA and protein expression were evaluated by real-time PCR and West- ern blot, respectively. SERCA2a activity was deter- mined by p-NPP method. Results Compared with control, cardiomyocytes in ISO group exhibited signifi- cantly decreased expression of SERCA2a both in mR- NA and protein levels. APS reversed these decreases in a dose-dependent manner. Furthermore, APS also significantly improved SERCA2a activity. Conclusions APS effectively buffers ISO induced downregulation of SERCA2a expression and activity. These effects ap- pear to mediate APS＇ s cardiovascular protection a- gainst Ca2 ＋ overload.

关 键 词：黄芪多糖 心肌细胞 异丙肾上腺素 Ca2+超载 SERCA2A 心肌保护 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]