芝麻素抗哮喘炎症作用研究  

Sesamin attenuates inflammation response in a murine model of asthma

作　　者：李良昌[1] 朴红梅[2] 延光海[1] 秦向征[1] 李光昭[1] 

机构地区：[1]延边大学医学院解剖学教研室,吉林延吉133002 [2]延边大学附属医院呼吸内科,吉林延吉133000

出　　处：《中国药理学通报》2015年第3期411-415,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81260665);吉林省科技厅项目(No 20130101121JC);吉林省教育厅项目(No吉教科合字2014第21号)

摘　　要：目的观察芝麻素抗哮喘作用,并探讨其可能的作用机制。方法 40只♂清洁级BALB/c小鼠,随机分为正常组、哮喘组、芝麻素低剂量组、芝麻素高剂量组和地塞米松组。体外卵清蛋白(OVA)诱导建立哮喘模型,ELISA和Western blot方法检测芝麻素对支气管炎肺泡灌洗液(BALF)和肺组织白细胞介素-4、5、13(IL-4、5、13)、干扰素-γ(IFN-γ)表达的影响。HE染色观察肺部炎症变化,Western blot方法检测IκBα磷酸化和NF-κB活化。结果哮喘组与正常组相比,炎症细胞计数、IL-4、IL-5、IL-13水平增高,而IFN-γ的表达明显降低(P<0.05),IκBα磷酸化和NF-κB核转位明显增加(P<0.05);芝麻素高剂量组明显降低炎症细胞计数、IL-4、IL-5、IL-13水平,提高IFN-γ的表达(P<0.05),同时抑制IκBα磷酸化和NF-κB核转位(P<0.05)。结论芝麻素通过抑制NF-κB激活,减轻哮喘炎症反应。Aim To investigate the effects of sesamin on inflammation response of asthma and to explore its possible mechanism of action. Methods Forty male BALB/c mice were randomly divided into five groups with 8 mice in each group： normal group, ovalbumin （ OVA） group, sesamin low dose group, sesamin high dose group and dexamethasone（ DXM） group. Asthma model mice were induced by OVA in vivo. The left lung was isolated for pathological examination. Experi-ment of ELISA and Western blot were used to deter-mine the effect of sesamin on IL-4 , IL-5 , IL-13 and IFN-γ expression. Hematoxylin and eosin stain was used to investigate pathological examination in lung tis-sue. Western blot was performed to detect the IκBαphosphorylation and NF-κB nuclear translocation. Re-sults The mice developed the following pathophysio-logical features of asthma： increased numbers of in- flammatory cells, increased levels of IL-4, IL-5 and IL-13 , decreased level of IFN-γ in bronchoalveolar lavage fluids （ BALF ） and lung tissues （ P 〈0. 05 ） , and increased IκBα phosphorylation and NF-κB nucle-ar translocation in lung tissues （ P 〈0. 05 ） . Adminis-tration of sesamin markedly reduced airway inflammato-ry cell recruitment, reduced the production of IL-4, IL-5 , IL-13 and increased IFN-γ in BALF and lung tissues（ P 〈0. 05 ） . The increased IκBα phosphoryla-tion and NF-κB nuclear translocation after OVA inhala-tion were inhibited by the administration of sesamin. Conclusion Sesamin attenuates inflammation re-sponse of asthma through suppression of NF-κB activa-tion.

关 键 词：哮喘 芝麻素 气道炎症 N-FκB 白介素4 白介素5 干扰素-Γ 

分 类 号：R-332[医药卫生] R284.1