二烯丙基二硫下调DJ-1抑制人白血病HL-60细胞增殖及诱导分化  

Diallyl Disulfide down regulated DJ-1 inhibiting ability of proliferation and inducing human leukemic cell differentiation

作　　者：王娟[1,2] 杨叶宁 秦晶[1] 唐玉娴[1] 李清叶[1] 苏琦[1] 谭晖[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]解放军总医院呼吸科,北京100853

出　　处：《中国药理学通报》2015年第3期416-420,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81100375);湖南省高校创新平台开放基金(No 11K057);南华大学博士科研启动基金(No 2010XQD40)

摘　　要：目的研究二烯丙基二硫(DADS)通过下调DJ-1对人白血病细胞系HL-60增殖抑制及诱导分化的影响。方法通过细胞形态法观察细胞分化;Western blot及免疫细胞化学检测DADS对HL-60细胞DJ-1表达的影响;针对DJ-1mRNA序列设计合成siRNA转染人白血病细胞HL-60,Western blot检测基因干扰效果;利用MTT法及间接免疫荧光检测DADS及DJ-1沉默对HL-60细胞的生长及细胞分化的影响。结果 DADS可以诱导HL-60细胞向成熟粒细胞系样分化。DADS呈时间依赖性抑制DJ-1的表达(P<0.05),DJ-1干扰组细胞生长减慢(P<0.05),与DADS共同作用后可诱导HL-60细胞分化。检测细胞生长情况发现干扰组细胞生长减慢(P<0.05)。结论 DADS通过下调DJ-1抑制细胞增殖并诱导HL-60细胞分化;干扰DJ-1基因可以增强DADS对HL-60细胞的增殖抑制诱导分化作用。Aim To observe the effect of DADS on the poliferation and differentiation of human leukemic cells by down-regulating DJ-1 . Methods Cell differentia-tion were detected by morphologic observation. The effects of DADS on DJ-1 expression in HL-60 cells were detected by immunocytochemistry and Western blot. DJ-1-specific siRNA was designed and synthe-sized. Small interfering RNA was transfected into HL-60 cells to knock down the DJ-1 expression. Western blot was used to detect the siRNA interference efficien-cy. The effects of DADS and DJ-1 silence on cell growth and differentiation in HL-60 cells were detected by MTT method and indirect immunofluorescence de-tection. Results HL-60 cells were induced differentia-tion toward neutrophilic by DADS. The expression of DJ-1 was inhibited in HL-60 cells after treated with DADS in a time dependent manner （ P 〈0. 05 ） . DJ-1 protein was overexpressed in HL-60 cell lines, which could be reversed after treated with DADS. Fluorescent-ly labeled DJ-1-siRNA transfected HL-60 cells. All these detections showed that the growth rate of DJ-1-siRNA group was lower （ P 〈 0. 05 ） . Conclusion DADS inhibits cell proliferation and induces cell differ-entiation by down-regulating DJ-1 in HL-60 cell lines. Silencing DJ-1 gene enhances the rate of inhibition of proliferation and induction differentiation of HL-60 cell lines after treated with DADS.

关 键 词：白血病 DJ-1 DADS 诱导分化 基因沉默 增殖 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]