氧载体对小白链霉菌发酵生产ε-聚赖氨酸的影响  

Effect of oxygen-vectors on the production of ε-poly-L-lysine

作　　者：薄芳芳[1,2] 许召贤[1,2] 孙朱贞 曹长虹[1,2] 夏军[1,2] 徐虹[1,2] 冯小海[1,2] 

机构地区：[1]材料化学工程国家重点实验室,江苏南京210009 [2]南京工业大学食品与轻工学院,江苏南京210009

出　　处：《生物工程学报》2015年第3期431-435,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.21176123);国家重点基础研究发展计划(973计划)(No.2013CB733603);高等学校博士学科点专项科研基金(No.20113221130001);材料化学工程国家重点实验室基金资助~~

摘　　要：为了有效改善发酵体系中的溶氧水平,提高小白链霉菌Streptomyces albulus PD-1发酵生产ε-聚赖氨酸的能力,文中通过对氧载体的种类、最佳添加浓度以及添加时间进行筛选,最终确定在0 h添加0.5%(V/V)的正十二烷促进ε-聚赖氨酸生产效果最佳。在5 L发酵罐0 h添加0.5%的正十二烷进行批次补料发酵,ε-聚赖氨酸的产量和菌体干重分别可以达到(30.8±0.46)g/L和(33.8±0.29)g/L,较之对照组分别提高了31.6%和20.7%。ε-聚赖氨酸的产量和菌体干重的提高归因于0.5%正十二烷的添加促进发酵液中溶氧水平从23.8%提高到32%,同时发酵液中的一种主要副产物(聚二氨基丙酸)的含量下降31%。实验结果表明,正十二烷的添加可以提高S.albulus PD-1发酵液中的溶氧水平,抑制副产物的生成,促进ε-聚赖氨酸的合成。To enhance the production of ε-poly-L-lysine（ε-PL） by improving dissolved oxygen level of the fermentation system, different oxygen-vectors were added to broth and n-dodecane was screened as the best oxygen-vector. The best amount of n-dodecane was 0.5%（V/V） and the best time was at start of the fermentation. In a fed-batch fermentation in a 5 L bioreactor, ε-PL concentration reached a maximum of（30.8±0.46） g/L and the dry cell weight obtained was（33.8±0.29） g/L, increasing by 31.6% and 20.7% compared with the control group, respectively. This improvement can be related to 0.5% n-dodecane could maintain dissolved oxygen concentration 32% of air concentration compared with 23.8% in ε-PL production phase, and the production of a main by-product, poly-L-diaminopropionic acid, fell by 31%. These results indicated that the dissolved oxygen level in the broth was improved by adding n-dodecane, which can inhibit the by-product production and improve the biosynthesis of ε-PL.

关 键 词：正十二烷 氧载体 Ε-聚赖氨酸 小白链霉菌PD-1 

分 类 号：Q936[生物学—微生物学]