乳腺癌中EZH2和p53蛋白表达及其临床意义  

Expression of EZH2 and p53 in breast cancer and their clinical significance

作　　者：汪小霞[1] 孟刚[1] 李丽[1] 王岳君[1,2] 杨苗苗[1,3] 韩彪[1] 陈青[1] 黄雯[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]安徽医科大学第四附属医院病理科,合肥230022 [3]安徽省第二人民医院病理科,合肥230041

出　　处：《临床与实验病理学杂志》2015年第3期273-276,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨乳腺癌组织中EZH2和p53蛋白的表达,分析二者表达与乳腺癌临床病理特征及预后的关系。方法采用免疫组化Max Vision法检测50例乳腺腺病、92例乳腺浸润性小叶癌(invasive lobular carcinoma,ILC)和200例乳腺浸润性导管癌(invasive ductal carcinoma,IDC)组织中EZH2和p53的表达及二者的相关性。结果 EZH2在ILC与IDC中的表达差异无统计学意义(P>0.016 7),其在乳腺腺病组织中的表达低于ILC与IDC组织(P<0.016 7)。EZH2表达与患者年龄、绝经状态、组织学类型、p TNM分期等均无关,与肿瘤直径、淋巴结转移、分子亚型、生存状态及p53表达有关(P<0.05)。p53蛋白在乳腺腺病和ILC组织中的表达差异无统计学意义(P>0.016 7),其在IDC组织中的表达高于ILC与乳腺腺病组织(P<0.0167)。p53表达与患者年龄、绝经状态、肿瘤直径、淋巴结转移等无关,与组织学类型、p TNM分期、分子亚型及生存状态相关(P<0.05)。Kaplan-Meier生存分析示:EZH2及p53蛋白表达与乳腺癌患者无病生存率及总生存率具有相关性(P<0.05);COX多因素回归分析显示:EZH2及p53蛋白表达是乳腺癌患者生存的独立影响因素。结论在乳腺腺病、ILC及IDC组织中,EZH2及p53蛋白表达逐渐升高,二者表达呈正相关。EZH2及p53蛋白表达水平对评价乳腺癌患者预后有重要价值。Purpose To investigate the expression of EZH2 and p53 protein in breast cancer and to analyze their relationship with the clinical pathologic characteristics and prognosis. Methods The expression of EZH2 and p53 protein were detected by immunohisto- chemical method in 50 cases of breast adenosis tissues, 92 cases of breast invasive lobular carcinoma （ILC） and 200 cases of breast in- vasive duetal carcinoma （IDC） , and their correlation was also analyzed. Results There was no statistical significance of EZH2 be- tween ILC and IDC （P 〉 0. 016 7）, while its expression in breast adenosis tissues was lower than that in ILC and IDC （P 〈 0. 016 7）. In breast cancer the expression of EZH2 protein were not correlated with patient age, menopausal status, histological types, and pTNM stage. In contrast, its expression correlated with the tumor size, lymph node metastasis, molecular subtype, survival status and p53 （ P 〈0. 05）. There was no statistical significance of p53 between ILC and breast adenosis tissues （P 〉0. 016 7） , while its expression in IDC was higher than that in ILC and breast adenosis tissues （P 〈 0. 016 7）. Its expression had no related to patient age, menopausal status, tumor size, lymph node metastasis in breast cancer, but related to histological types, pTNM stage, molecular subtype and sur- vival status （ P 〈 0. 05 ）. The Kaplan-Meier survival analysis showed the expression of EZH2 and p53 had correlated with disease-free and overall survival rates of breast cancer （ P 〈 0. 05 ）. Multivariate COX regression analysis showed that the expression of EZH2 and p53 were independent affecting factors to breast cancer patients. Conclusion The expression of EZH2 and p53 protein increase in the breast adenosis, ILC and IDC gradually, and they have positive correlation. The expression levels of EZH2 and p53 protein have im- portant value to evaluate the prognosis of breast cancer patients.

关 键 词：乳腺肿瘤 浸润性导管癌 浸润性小叶癌 EZH2 P53 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]