癫痫大鼠海马组织长链非编码RNA差异表达的研究  

Long non-coding RNA expression profile of the hippocampus in a rat epilepsy model

作　　者：韩春雷[1] 孟凡刚[1] 刘阳[1] 王开亮[1] 赵学敏[1] 张鑫[1] 张建国[2] 

机构地区：[1]首都医科大学北京市神经外科研究所,100050 [2]首都医科大学附属北京天坛医院神经外科、神经电刺激研究与治疗北京市重点实验室

出　　处：《中华神经外科杂志》2015年第3期304-308,共5页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（81471315）;首都医科大学基础一临床科研合作基金（1140170045-14JL52）;北京市医院管理局重点医学专业发展计划,癫痫与功能神经外科（ZYLX201305）;北京市金桥工程种子资金

摘　　要：目的 利用基因芯片筛选癫痫大鼠海马组织中差异表达的长链非编码RNA（lncRNAs）和mRNAs,分析lncRNAs在癫痫发病中的可能作用.方法 建立癫痫大鼠模型,提取海马RNA,利用Rat Gene 2.0 ST微阵列芯片分别检测5例癫痫及5例健康大鼠海马组织的lncRNAs和mRNAs表达,对差异表达的mRNAs进行GO、Pathway分析,构建lncRNAs和mRNAs的共表达网络,预测lncRNAs的可能功能.结果 按癫痫组与对照组的基因转录产物表达倍数大于1.2并且统计量P ＜0.05为标准筛选,得到差异lncRNAs 198个（上调92个,下调106个）,差异表达mRNAs 1 804个（上调983个,下调821个）.差异表达的mRNAs涉及离子转运、缺氧反应、细胞粘附、炎性反应等功能.Pathway分析显示,差异表达mRNAs在MAPK信号通路、局部粘附、p53信号通路、凋亡等方面功能的基因有显著地变化.生物信息学分析可初步预测差异lncRNAs可能的生物学功能及作用靶点.结论 本研究发现了癫痫大鼠海马组织中差异表达的lncRNAs和mRNAs,lncRNAs可能通过调控mRNAs的表达参与癫痫的发病或发展.Objective To detect differentially expressed lncRNAs and mRNAs in hippocampus from epileptic rats using microarray and explore the role of lncRNAs in the pathogenesis of epilepsy.Methods The lithium-pilocarpine-induced status epilepticus model was established in Sprague-Dawley rats.Total RNA samples were isolated from hippocampus of 5 epileptic rats and 5 normal rats 24 hours after the induction of status epilepticus.Rat Gene 2.0 ST microarray was used to detect deregulated lncRNAs and mRNAs in the hippocampus.GO and Pathway analysis was performed.The coding-noncoding gene co-expression network was established.Results A total of 198 deregulated lncRNAs and 1 804 deregulated mRNAs were detected in epileptic rats.GO Term enrichment in the differentially expressed mRNAs list included ion transport,response to hydrogen peroxide,cell adhesion,inflammatory response etc.Differentially expressed mRNAs might involve in MAPK signaling pathway,focal adhesion,p53 signaling pathway,apoptosis,etc.Target regulated by lncRNAs was predicted with bioinformatic prediction.Conclusions This study explored the lncRNAs and mRNAs expression in epileptic rats using microarray.Differentially expressed lncRNAs might play a role in the pathogenesis of temporal lobe epilepsy.

关 键 词：癫痫 大鼠 基因表达谱 长链非编码RNA 

分 类 号：R742.1[医药卫生—神经病学与精神病学]