徐州地区女性宫颈细胞中人乳头瘤病毒感染基因型的分析  

Genotypes analysis of HPV infection in cervical cell samples among women in Xxuzhou region

作　　者：夏林[1] 龚培尧[2] 耿建祥[2] 王宏景[2] 谭进[2] 肖蔚[2] 马文元[2] 龙秀荣[2] 赵雪[2] 

机构地区：[1]江苏省扬中市人民医院病理科,江苏杨中212200 [2]南京中医药大学第三附属医院病理科/江苏省HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2015年第7期882-884,共3页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项资助项目(2009-92)

摘　　要：目的本文旨在探讨徐州地区女性宫颈细胞中人乳头瘤病毒(HPV)感染基因谱的分布情况及其临床意义。方法从8 010份徐州地区宫颈细胞标本中提取23种HPV DNA,使用聚合酶链式反应和反向点杂交相结合的基因芯片技术对宫颈细胞23种HPV型别进行测定,同时分析受检者的相关资料。结果 8 010例女性中有1 852例HPV感染者,总的HPV感染率为23.12%,其阳性检出率一重型别为17.17%,一重中最主要感染型别是HPV16型为4.35%,其次为HPV58型2.12%、52型1.82%。阳性检出率多重HPV感染为5.96%;其中16+58型、16+52型、11+16型,分别占多重感染的4.40%、2.94%和2.52%,是多重感染的主要型别。结论一重HPV16、58和52型及多重16+58、16+52和11+16型是感染徐州地区女性宫颈细胞的主要基因型,此基因芯片检测技术适用于宫颈细胞标本,一次可检测23种HPV基因型,其特异性强,敏感性高,对我国女性宫颈HPV感染基因型分布的分子流行病学调查研究具有重要的意义。Objective To investigate the distribution situation of human papillomavirus（HPV）genotypes profile in cervical cells among women in Xuzhou area and its clinical significance.Methods 23 kinds of HPV DNA were extracted in cervical cell samples from 8 010 women in Xiuzhou area.The gene-chips technique of PCR combined with reverse dot blot was adopted to detect the HPV genotypes.Results Among 8010 cervical cell samples,there were 1 852 HPV infected cases,the total HPV infection rate was23.12%,the HPV infection rates of single type accounted for 17.17% and its predominant types were 16type（4.35%）,followed by 58type（2.12%）and 52type（1.82%）,The detection rate of multiple HPV infection was 5.96%,in which the predominant types were HPV16＋58（4.40%）,16＋52（2.94%）,11＋16（2.52%）.Conclusion The single HPV infection of HPV16,58,52 and the multiple HPV infection of HPV16＋58,16＋52,11＋16are the main genotypes of cervical cells among women in Xuzhou area,this gene chip technique is suitable for the cervical cell sample,its once detection can detect 23 kinds of HPV genotypes with high specificity and high sensitivity,which has an important significance for the molecular epidemiologic survey study of HPV genotypes distribution among women in our country.

关 键 词：女性 宫颈细胞 人乳头瘤病毒 基因分型 基因芯片技术 

分 类 号：R737.33[医药卫生—肿瘤]