人乳头状瘤病毒HPV感染的临床实验结果分析  

Analysis on results of clinical trials of HPV infection

作　　者：冯程娟[1,2] 苏东梅[1,2] 刘丽娥 黄颜清 

机构地区：[1]广东省茂名市中医院 [2]广州中医药大学附属茂名中医院检验科,广东茂名525000 [3]广州中医药大学附属茂名中医院病理科,广东茂名525000

出　　处：《国际检验医学杂志》2015年第7期903-905,共3页International Journal of Laboratory Medicine

摘　　要：目的探究感染了人乳头状瘤病毒(HPV)人群与疾病发生的相关性情况,为人们认识此疾病提供依据及一些合理建议。方法使用PCR-反向点杂交法分别对2012年8月至2013年8月来本院进行就诊的2 508例可疑患者进行了23种HPV基因亚型检测,包括18种高危亚型(HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83和MM4)和5种低危亚型(HPV6、11、42、43和44)。并且通过医院信息管理系统及检验科信息系统对病例进行病历信息追踪。结果HPV检出率为12.26%,其中高危型以HPV52最多见,占阳性比12.66%,其次为高危型HPV58、16型。低危型主要以43、6、11型较多见,占阳性比分别为14.61%、10.39%和8.44%。追踪观察阳性患者的转归情况,感染HPV52、16型者,两年内复查结果反复出现同型阳性现象。追踪分析了157例高危型(分别是52、58、16、18、66、33、56和68型别)HPV感染患者的TCT(膜式液基薄层细胞学检查)结果,高危型HPV感染与CINⅠ、CINⅡ、癌变病变呈密切相关性。追踪感染者的白带常规结果,表现为轻到中度炎症。结论高危型HPV亚型感染与宫颈病理学CINⅠ、CINⅡ、癌变密切相关。而白带常规显示为轻、中度的炎症表现,因此患者只进行简单的白带常规分析,已不能真实反映患者感染状态,应寻求与上级医院合作的方法,及时检测HPV感染情况,并对HPV阳性者进行定期的跟踪,是防治宫颈上皮内瘤变(CIN)与宫颈癌的有效途径。Objective To explore the relationship between human papilloma virus（HPV）infected population and disease occurrence in order to provide evidence and some reasonable suggestions for people understanding this disease.Methods A total of 2 508 suspected patients in our hospital from August 2012 to August 2013 were performed the HPV subtype detection by using PCR-reverse dot blot（RDB）,including 18 kinds of high risk HPV subtypes（HPV16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83,MM4）and 5kinds of low risk HPV subtypes（HPV6,11,42,43,44）.Finally,the patients condition was tracked through the hospital information management system and the clinical laboratory information systems.Results The HPV detection rate was12.26%,in which the high risk type was mainly HPV 52,accounting for 12.66%,followed by HPV 58,16.The low risk types were mainly HPV 43,6,11,accounting for 14.61%,10.39% and 8.44%respectively.By the follow up observation of clinical outcomes,the homotype positive phenomena were repeatedly appeared in the patients infected by HPV 52,16 within 2years.At the same time,the thinprep cytologist test（TCT）results in 157 cases of high risk HPV infection（genotype 52,58,16,18,66,33,56,68）were performed the tracking analysis,indicating that high risk HPV infection was closely associated with cervical intraepithelial neoplasia（CIN）Ⅰ,CIN Ⅱ and canceration.Meanwhile,their leucorrhea routine detection were followed up,which manifested by mild to moderate inflammation.Conclusion High risk HPV subtype infection is closely associated with CINⅠ,CIN Ⅱ and canceration,while the leucorrhea routine shows mild,moderate inflammation.Therefore,only conducting the simple leucorrhea routine analysis can not really reflect the infection status,it is necessary to look for the ways to cooperate with the superior hospital for timely detecting HPV infection situation,moreover regular follow up of people with HPV positive is the effective pathway to prevent CIN and cervical cancer.

关 键 词：人乳头状瘤病毒 宫颈上皮内瘤变 白带常规 

分 类 号：R737.33[医药卫生—肿瘤]